ArticleMolecular microbiology2013
Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis in Escherichia coli.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Cryo-EM reveals multiple mechanisms of ribosome inhibition by doxycycline.Nature communications · 2026Article
- Protein-Protein Interactions as Promising Molecular Targets for Novel Antimicrobials Aimed at Gram-Negative Bacteria.International journal of molecular sciences · 2025Review
- Emerging Quantitative Biochemical, Structural, and Biophysical Methods for Studying Ribosome and Protein-RNA Complex Assembly.Biomolecules · 2023Review
- Differential Proteomic Analysis ofBiology · 2022Article
- Review
- A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity Across the Tree of Life.Methods in molecular biology (Clifton, N.J.) · 2022Review
- RNase III, Ribosome Biogenesis and Beyond.Microorganisms · 2021Review
- Uncovering a delicate balance between endonuclease RNase III and ribosomal protein S15 in E. coli ribosome assembly.Biochimie · 2021Article
- A roadmap for rRNA folding and assembly during transcription.Trends in biochemical sciences · 2021Review
- Transcription complexes as RNA chaperones.Transcription · 2021Article
- Modulators of protein-protein interactions as antimicrobial agents.RSC chemical biology · 2021Review
- Article
- Article
- SuhB is an integral part of the ribosomal antitermination complex and interacts with NusA.Nucleic acids research · 2019Article
- Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis.Nucleic acids research · 2019Article
- Translation initiation factor IF2 contributes to ribosome assembly and maturation during cold adaptation.Nucleic acids research · 2019Article
- Transcriptional and post-transcriptional events trigger de novo infB expression in cold stressed Escherichia coli.Nucleic acids research · 2019Article
- Escherichia coli transcription factor NusG binds to 70S ribosomes.Molecular microbiology · 2018Article
- Extrachromosomal Nucleolus-Like Compartmentalization by a Plasmid-Borne Ribosomal RNA Operon and Its Role in Nucleoid Compaction.Frontiers in microbiology · 2018Article
- Identification of regulatory targets for the bacterial Nus factor complex.Nature communications · 2017Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
Abstract
Escherichia coli NusA and NusB proteins bind specific sites, such as those in the leader and spacer sequences that flank the 16S region of the ribosomal RNA transcript, forming a complex with RNA polymerase that suppresses Rho-dependent transcription termination. Although antitermination has long been the accepted role for Nus factors in rRNA synthesis, we propose that another major role for the Nus-modified transcription complex in rrn operons is as an RNA chaperone insuring co-ordination of 16S rRNA folding and RNase III processing that results in production of proper 30S ribosome subunits. This contrarian proposal is based on our studies of nusA and nusB cold-sensitive mutations that have altered translation and at low temperature accumulate 30S subunit precursors. Both phenotypes are suppressed by deletion of RNase III. We argue that these results are consistent with the idea that the nus mutations cause altered rRNA folding that leads to abnormal 30S subunits and slow translation. According to this idea, functional Nus proteins stabilize an RNA loop between their binding sites in the 5' RNA leader and on the transcribing RNA polymerase, providing a topological constraint on the RNA that aids normal rRNA folding and processing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.