ArticlePloS one2012
Sequence of two plasmids from Clostridium perfringens chicken necrotic enteritis isolates and comparison with C. perfringens conjugative plasmids.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- First genomic analysis of aFrontiers in microbiology · 2025Article
- Genomic Diversity and Virulence Factors ofMicroorganisms · 2024Article
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- A Poultry Subclinical Necrotic Enteritis Disease Model Based on NaturalFrontiers in physiology · 2022Article
- Role ofVaccines · 2021Review
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- Pathogenicity and virulence ofVirulence · 2021Article
- A Rapid and Simple Assay Correlates In Vitro NetB Activity withMicroorganisms · 2021Article
- Complete genomic sequence and analysis of β2 toxin gene mapping of Clostridium perfringens JXJA17 isolated from piglets in China.Scientific reports · 2021Article
- NetF-producingJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Review
- Virulence Plasmids of the Pathogenic Clostridia.Microbiology spectrum · 2019Article
- Review
- Whole genome analysis reveals the diversity and evolutionary relationships between necrotic enteritis-causing strains of Clostridium perfringens.BMC genomics · 2018Article
- Mechanisms of Action and Cell Death Associated withToxins · 2018Review
- Commentary: Probing Genomic Aspects of the Multi-Host PathogenFrontiers in microbiology · 2018Article
- Experimental induction of necrotic enteritis in chickens by a netB-positive Japanese isolate of Clostridium perfringens.The Journal of veterinary medical science · 2017Article
- Recurring Necrotic Enteritis Outbreaks in Commercial Broiler Chicken Flocks Strongly Influence Toxin Gene Carriage and Species Richness in the ResidentFrontiers in microbiology · 2017Article
- Influence of pCP1NetB ancillary genes on the virulence ofGut pathogens · 2017Article
- Comparative transcriptome analysis by RNAseq of necrotic enteritis Clostridium perfringens during in vivo colonization and in vitro conditions.BMC microbiology · 2016Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Twenty-six isolates of Clostridium perfringens of different MLST types from chickens with necrotic enteritis (NE) (15 netB-positive) or from healthy chickens (6 netB-positive, 5 netB-negative) were found to contain 1-4 large plasmids, with most netB-positive isolates containing 3 large and variably sized plasmids which were more numerous and larger than plasmids in netB-negative isolates. NetB and cpb2 were found on different plasmids consistent with previous studies. The pathogenicity locus NELoc1, which includes netB, was largely conserved in these plasmids whereas NeLoc3, present in the cpb2 containing plasmids, was less well conserved. A netB-positive and a cpb2-positive plasmid were likely to be conjugative, and the plasmids were completely sequenced. Both plasmids possessed the intact tcp conjugative region characteristic of C. perfringens conjugative plasmids. Comparative genomic analysis of nine CpCPs, including the two plasmids described here, showed extensive gene rearrangements including pathogenicity locus and accessory gene insertions around rather than within the backbone region. The pattern that emerges from this analysis is that the major toxin-containing regions of the variety of virulence-associated CpCPs are organized as complex pathogenicity loci. How these different but related CpCPs can co-exist in the same host has been an unanswered question. Analysis of the replication-partition region of these plasmids suggests that this region controls plasmid incompatibility, and that CpCPs can be grouped into at least four incompatibility groups.
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