ArticleThe Journal of biological chemistry2013
The biochemical mechanism of auxin biosynthesis by an arabidopsis YUCCA flavin-containing monooxygenase.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers, 1 of them a synthesis that pooled it.
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Who cites it
92 citing papers in PubMed, 1 synthesis or guideline pooled it, 201 citations in OpenAlex.
- Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks.International journal of molecular sciences · 2018Pooled it
- Cell Signaling by Tryptophan Catabolism.Biochemistry · 2026Review
- Unexpected Dual Function of Plant YUCCA Enzymes Links Chlorophyll Catabolism to Auxin Homeostasis.Angewandte Chemie (International ed. in English) · 2026Article
- Genome-Wide Analysis of thePlants (Basel, Switzerland) · 2026Article
- The auxin gatekeepers: Evolution and diversification of the YUCCA family.The Plant journal : for cell and molecular biology · 2025Article
- Indole-3-Butyric Acid (IBA) Derivatives for Root Architecture Modulation: Potent Promoters viaACS omega · 2025Article
- Auxin homeostasis: new roads in a tight network.Journal of experimental botany · 2025Article
- Transcriptomic profiles reveal hormonal regulation of sugar-induced stolon initiation in potato.Scientific reports · 2025Article
- Auxin and tryptophan trigger common responses in the streptophyte alga Penium margaritaceum.Current biology : CB · 2025Article
- Plant Signaling Hormones and Transcription Factors: Key Regulators of Plant Responses to Growth, Development, and Stress.Plants (Basel, Switzerland) · 2025Review
- Exploring Metabolic Pathways and Gene Mining During Cotton Flower Bud Differentiation Stages Based on Transcriptomics and Metabolomics.International journal of molecular sciences · 2025Article
- Dressed Up to the Nines: The Interplay of Phytohormones Signaling and Redox Metabolism During Plant Response to Drought.Plants (Basel, Switzerland) · 2025Review
- Nitrate Starvation Induces Lateral Root Organogenesis inInternational journal of molecular sciences · 2024Article
- Auxin and carbohydrate control flower bud development in Anthurium andraeanum during early stage of sexual reproduction.BMC plant biology · 2024Article
- Genetics and metabolic responses of Artemisia annua L to the lake of phosphorus under the sparingly soluble phosphorus fertilizer: evidence from transcriptomics analysis.Functional & integrative genomics · 2024Article
- Investigating the biosynthesis and roles of the auxin phenylacetic acid duringFrontiers in plant science · 2024Article
- Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses inPlants (Basel, Switzerland) · 2023Article
- Recent advances in auxin biosynthesis and homeostasis.3 Biotech · 2023Review
- Precise Regulation of the TAA1/TAR-YUCCA Auxin Biosynthesis Pathway in Plants.International journal of molecular sciences · 2023Review
- The birth of a giant: evolutionary insights into the origin of auxin responses in plants.The EMBO journal · 2023Review
32 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Auxin regulates every aspect of plant growth and development. Previous genetic studies demonstrated that YUCCA (YUC) flavin-containing monooxygenases (FMOs) catalyze a rate-limiting step in auxin biosynthesis and that YUCs are essential for many developmental processes. We proposed that YUCs convert indole-3-pyruvate (IPA) to indole-3-acetate (IAA). However, the exact biochemical mechanism of YUCs has remained elusive. Here we present the biochemical characterization of recombinant Arabidopsis YUC6. Expressed in and purified from Escherichia coli, YUC6 contains FAD as a cofactor, which has peaks at 448 nm and 376 nm in the UV-visible spectrum. We show that YUC6 uses NADPH and oxygen to convert IPA to IAA. The first step of the YUC6-catalyzed reaction is the reduction of the FAD cofactor to FADH(-) by NADPH. Subsequently, FADH(-) reacts with oxygen to form a flavin-C4a-(hydro)peroxy intermediate, which we show has a maximum absorbance at 381 nm in its UV-visible spectrum. The final chemical step is the reaction of the C4a-intermediate with IPA to produce IAA. Although the sequences of the YUC enzymes are related to those of the mammalian FMOs, which oxygenate nucleophilic substrates, YUC6 oxygenates an electrophilic substrate (IPA). Nevertheless, both classes of enzymes form quasi-stable C4a-(hydro)peroxyl FAD intermediates. The YUC6 intermediate has a half-life of ∼20 s whereas that of some FMOs is >30 min. This work reveals the catalytic mechanism of the first known plant flavin monooxygenase and provides a foundation for further investigating how YUC activities are regulated in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.