ArticleRNA (New York, N.Y.)2013
mRNAs containing the histone 3' stem-loop are degraded primarily by decapping mediated by oligouridylation of the 3' end.
Article in RNA (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Oligo(uridine) tailing and exonucleolytic decay drive 40S ribosome degradation in response to stress.Molecular cell · 2026Article
- A tale of non-canonical tails: gene regulation by post-transcriptional RNA tailing.Nature reviews. Molecular cell biology · 2020Review
- UPFront and center in RNA decay: UPF1 in nonsense-mediated mRNA decay and beyond.RNA (New York, N.Y.) · 2019Review
- Structural basis for acceptor RNA substrate selectivity of the 3' terminal uridylyl transferase Tailor.Nucleic acids research · 2019Article
- The balance of poly(U) polymerase activity ensures germline identity, survival and development inDevelopment (Cambridge, England) · 2018Article
- 3' RNA Uridylation in Epitranscriptomics, Gene Regulation, and Disease.Frontiers in molecular biosciences · 2018Review
- Use of Mature miRNA Strand Selection in miRNAs Families in Cervical Cancer Development.International journal of molecular sciences · 2017Review
- Molecular basis for cytoplasmic RNA surveillance by uridylation-triggered decay in Drosophila.The EMBO journal · 2016Article
- TUT7 catalyzes the uridylation of the 3' end for rapid degradation of histone mRNA.RNA (New York, N.Y.) · 2016Article
- TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs.The EMBO journal · 2016Article
- Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex.RNA (New York, N.Y.) · 2016Article
- Oligoadenylation of 3' decay intermediates promotes cytoplasmic mRNA degradation in Drosophila cells.RNA (New York, N.Y.) · 2016Article
- Uridylation and adenylation of RNAs.Science China. Life sciences · 2015Review
- EnD-Seq and AppEnD: sequencing 3' ends to identify nontemplated tails and degradation intermediates.RNA (New York, N.Y.) · 2015Article
- Article
- The mRNP remodeling mediated by UPF1 promotes rapid degradation of replication-dependent histone mRNA.Nucleic acids research · 2014Article
- Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes.Molecular cell · 2014Article
- The C-terminal extension of Lsm4 interacts directly with the 3' end of the histone mRNP and is required for efficient histone mRNA degradation.RNA (New York, N.Y.) · 2014Article
- Proteins involved in the degradation of cytoplasmic mRNA in the major eukaryotic model systems.RNA biology · 2014Review
- Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Metazoan replication-dependent histone mRNAs are only present in S-phase, due partly to changes in their stability. These mRNAs end in a unique stem-loop (SL) that is required for both translation and cell-cycle regulation. Previous studies showed that histone mRNA degradation occurs through both 5'→3' and 3'→5' processes, but the relative contributions are not known. The 3' end of histone mRNA is oligouridylated during its degradation, although it is not known whether this is an essential step. We introduced firefly luciferase reporter mRNAs containing the histone 3' UTR SL (Luc-SL) and either a normal or hDcp2-resistant cap into S-phase HeLa cells. Both mRNAs were translated, and translation initially protected the mRNAs from degradation, but there was a lag of ∼40 min with the uncleavable cap compared to ∼8 min for the normal cap before rapid decay. Knockdown of hDcp2 resulted in a similar longer lag for Luc-SL containing a normal cap, indicating that 5'→3' decay is important in this system. Inhibition of DNA replication with hydroxyurea accelerated the degradation of Luc-SL. Knockdown of terminal uridyltransferase (TUTase) 4 but not TUTase 3 slowed the decay process, but TUTase 4 knockdown had no effect on destabilization of the mRNA by hydroxyurea. Both Luc-SL and its 5' decay intermediates were oligouridylated. Preventing oligouridylation by 3'-deoxyadenosine (cordycepin) addition to the mRNA slowed degradation, in the presence or absence of hydroxyurea, suggesting oligouridylation initiates degradation. The spectrum of oligouridylated fragments suggests the 3'→5' degradation machinery stalls during initial degradation, whereupon reuridylation occurs.
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