ArticleOsteoarthritis and cartilage2013
Effects of doxycycline on mesenchymal stem cell chondrogenesis and cartilage repair.
Article in Osteoarthritis and cartilage, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Tetracycline use in treating osteoarthritis: a systematic review.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2021Pooled it
- A specific protocol of autologous bone marrow concentrate and platelet products versus exercise therapy for symptomatic knee osteoarthritis: a randomized controlled trial with 2 year follow-up.Journal of translational medicine · 2018Trial
- Diffuse Synovial Lipomatosis and Meniscal Hypoplasia in an Adolescent Patient: A Case Report.Cureus · 2026Article
- Case Report: Equine allogeneic umbilical cord blood mesenchymal stromal cells (CB-MSC) as adjunctive therapy in a foal with septic arthritis and osteomyelitis.Frontiers in veterinary science · 2026Article
- Overexpression of BDNF and uPA Combined with the Suppression of Von Hippel-Lindau Tumor Suppressor Enhances the Neuroprotective Activity of the Secretome of Human Mesenchymal Stromal Cells in the Model of Intracerebral Hemorrhage.International journal of molecular sciences · 2025Article
- Bone Mesenchymal Stromal Cell-Derived Extracellular Vesicles Protect Articular Cartilage Through Regulating tRF-Gln-TTG-019/UBL3.Mediators of inflammation · 2025Article
- Cartilage repair using stem cells & biomaterials: advancement from bench to bedside.Molecular biology reports · 2020Review
- Doxycycline preserves chondrocyte viability and function in human and calf articular cartilage ex vivo.Physiological reports · 2020Article
- Direct conversion of human fibroblasts into therapeutically active vascular wall-typical mesenchymal stem cells.Cellular and molecular life sciences : CMLS · 2020Article
- Mesenchymal Stromal Cells as Potential Antimicrobial for Veterinary Use-A Comprehensive Review.Frontiers in microbiology · 2020Review
- Effects of various antimicrobial agents on multi-directional differentiation potential of bone marrow-derived mesenchymal stem cells.World journal of stem cells · 2019Review
- Mesenchymal Stem Cell Therapy for Osteoarthritis: The Critical Role of the Cell Secretome.Frontiers in bioengineering and biotechnology · 2019Review
- Article
- Doxycycline enhances survival and self-renewal of human pluripotent stem cells.Stem cell reports · 2014Article
- Effect of Ermiao Recipe with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats.Chinese journal of integrative medicine · 2014Article
- Tissue-engineered cartilage with inducible and tunable immunomodulatory properties.Biomaterials · 2014Article
- Persistence, localization, and external control of transgene expression after single injection of adeno-associated virus into injured joints.Human gene therapy · 2013Article
- Controlled spatial and temporal release of neurotrophic growth factor from genetically modified tissue engineering living scaffolds.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
objectiveStrategies to improve cartilage repair tissue quality after bone marrow cell-based procedures may reduce later development of osteoarthritis. Doxycycline is inexpensive, well-tolerated, and has been shown to reduce matrix-metalloproteinases (MMPs) and osteoarthritis progression. This study tests the hypotheses that doxycycline reduces MMP, enhances chondrogenesis of human bone marrow-derived mesenchymal stem cells (hMSC), and improves in vivo cartilage repair.
designNinety hMSC pellets were cultured in chondrogenic media with either 0-, 1- or 2-μg/mL doxycycline. Pellets were evaluated with stereomicroscopy, proteoglycan assay, qRT-PCR, and histology. Osteochondral defects (OCDs) were created in the trochlear grooves of 24-Sprague-Dawley rats treated with/without oral doxycycline. Rats were sacrificed at 12-weeks and repair tissues were examined grossly and histologically.
resultshMSC pellets with 1-μg/mL (P = 0.014) and 2-μg/mL (P = 0.002) doxycycline had larger areas than pellets without doxycycline. hMSC pellets with 2-μg/mL doxycycline showed reduced mmp-13 mRNA (P = 0.010) and protein at 21-days. Proteoglycan, DNA contents, and mRNA expressions of chondrogenic genes were similar (P > 0.05). For the in vivo study, while the histological scores were similar between the two groups (P = 0.116), the gross scores of the OCD repair tissues in doxycycline-treated rats were higher at 12-weeks (P = 0.017), reflective of improved repair quality. The doxycycline-treated repairs also showed lower MMP-13 protein (P = 0.029).
conclusionsThis study shows that doxycycline improves hMSC chondrogenesis and decreases MMP-13 in pellet cultures and within rat OCDs. Doxycycline exerted no negative effect on multiple measures of chondrogenesis and cartilage repair. These data support potential use of doxycycline to improve cartilage repair to delay the onset of osteoarthritis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.