Evidence map›Paper›PMID 23185643›Full record

ArticlePloS one2012

Time-gated optical projection tomography allows visualization of adult zebrafish internal structures.

Luca Fieramonti, Andrea Bassi, Efrem Alessandro Foglia, Anna Pistocchi, Cosimo D'Andrea, Gianluca Valentini, Rinaldo Cubeddu, Sandro De Silvestri, Giulio Cerullo, Franco Cotelli

Abstract read
In one paragraph

Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

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  15. Danio rerio: the Janus of the bone from embryo to scale.Clinical cases in mineral and bone metabolism : the official journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Luca FieramontiDepartment of Physics, Politecnico di Milano, Milano, Italy.
Andrea Bassi
Efrem Alessandro Foglia
Anna Pistocchi
Cosimo D'Andrea
Gianluca Valentini
Rinaldo Cubeddu
Sandro De Silvestri
Giulio Cerullo
Franco Cotelli

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Optical imaging through biological samples is compromised by tissue scattering and currently various approaches aim to overcome this limitation. In this paper we demonstrate that an all optical technique, based on non-linear upconversion of infrared ultrashort laser pulses and on multiple view acquisition, allows the reduction of scattering effects in tomographic imaging. This technique, namely Time-Gated Optical Projection Tomography (TGOPT), is used to reconstruct three dimensionally the internal structure of adult zebrafish without staining or clearing agents. This method extends the use of Optical Projection Tomography to optically diffusive samples yielding reconstructions with reduced artifacts, increased contrast and improved resolution with respect to those obtained with non-gated techniques. The paper shows that TGOPT is particularly suited for imaging the skeletal system and nervous structures of adult zebrafish.

Indexed as

ArtifactsAnimalsAnimals, Genetically ModifiedBone and BonesImaging, Three-DimensionalLasersLightNervous SystemTime FactorsTomography, OpticalZebrafish

Identifiers

PMID23185643
PMCPMC3501464

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.