ArticlePloS one2012
Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 183 citations in OpenAlex.
- Cell-type-specific glutamine synthetase inhibition reveals the intercellular nitrogen economy in Anabaena sp. PCC 7120.Plant physiology · 2026Article
- Employing Nanotechnology to Enhance Ethanol Production in Two Synechococcus elongatus Strains.Chembiochem : a European journal of chemical biology · 2026Article
- Biocatalytic membrane for membrane inlet mass spectrometry.Scientific reports · 2026Article
- Cyanobacterial Biofertilizer Production by Guanidine-Producing Enzymes.ACS synthetic biology · 2026Review
- CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.BMC biotechnology · 2026Article
- Deciphering guanidine assimilation and riboswitch-based gene regulation in cyanobacteria for synthetic biology applications.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Experimental Evidence for Radical-Polar Crossover in Competition with, Rather Than Preceding, Alkene Formation by Microbial Ethylene-Forming Enzyme.Journal of the American Chemical Society · 2025Article
- Heterologous Production of the Structurally Complex Diterpenoid Forskolin inBioengineering (Basel, Switzerland) · 2025Article
- Thylakoid Targeting Improves Stability of a Cytochrome P450 in the CyanobacteriumACS synthetic biology · 2025Article
- Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology.Synthetic and systems biotechnology · 2025Review
- Testing eukaryotic routes for heterologous production of ketocarotenoids in cyanobacteria.Sustainable microbiology · 2025Article
- Harnessing the potential of microalgae for the production of monoclonal antibodies and other recombinant proteins.Protoplasma · 2024Review
- Hydroponics with Microalgae and Cyanobacteria: Emerging Trends and Opportunities in Modern Agriculture.Biotech (Basel (Switzerland)) · 2024Review
- Article
- Evolution of ethylene as an abiotic stress hormone in streptophytes.Environmental and experimental botany · 2023Review
- Construction of pSM201v: A broad host range replicative vector based on shortening of RSF1010.Heliyon · 2023Article
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Discovery of Five New Ethylene-Forming Enzymes for Clean Production of Ethylene inInternational journal of molecular sciences · 2022Article
- Development of a Highly Sensitive Luciferase-Based Reporter System To Study Two-Step Protein Secretion in Cyanobacteria.Journal of bacteriology · 2022Article
- Cross-Activation of Two Nitrogenase Gene Clusters by CnfR1 or CnfR2 in the Cyanobacterium Anabaena variabilis.Microbiology spectrum · 2021Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ethylene-forming enzyme (EFE) from Pseudomonas syringae catalyzes the synthesis of ethylene which can be easily detected in the headspace of closed cultures. A synthetic codon-optimized gene encoding N-terminal His-tagged EFE (EFEh) was expressed in Synechocystis sp. PCC 6803 (Synechocystis) and Escherichia coli (E. coli) under the control of diverse promoters in a self-replicating broad host-range plasmid. Ethylene synthesis was stably maintained in both organisms in contrast to earlier work in Synechococcus elongatus PCC 7942. The rate of ethylene accumulation was used as a reporter for protein expression in order to assess promoter strength and inducibility with the different expression systems. Several metal-inducible cyanobacterial promoters did not function in E. coli but were well-regulated in cyanobacteria, albeit at a low level of expression. The E. coli promoter P(trc) resulted in constitutive expression in cyanobacteria regardless of whether IPTG was added or not. In contrast, a Lac promoter variant, P(A1lacO-1), induced EFE-expression in Synechocystis at a level of expression as high as the Trc promoter and allowed a fine level of IPTG-dependent regulation of protein-expression. The regulation was tight at low cell density and became more relaxed in more dense cultures. A synthetic quorum-sensing promoter system was also constructed and shown to function well in E. coli, however, only a very low level of EFE-activity was observed in Synechocystis, independent of cell density.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.