ArticlePloS one2012
Molecular characterization of transgene integration by next-generation sequencing in transgenic cattle.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Read-mapping signatures for molecular characterization of transgenic DNA insertions in paired-end Illumina data.BMC biotechnology · 2026Article
- Breeding and Molecular Characterization of Insect-Resistant Transgenic Cotton.Plants (Basel, Switzerland) · 2026Article
- Unbiased Long-Read Whole-Genome Sequencing Enables High-Resolution Mapping of Transgene Concatenation and Off-target Genomic Disruption in a Mouse Model.Computational and structural biotechnology journal · 2026Article
- Transgene Mapping in Animals: What to Choose?International journal of molecular sciences · 2025Review
- Review of the technology used for structural characterization of the GMO genome using NGS data.Genomics & informatics · 2024Article
- Development and evaluation of triple gene transgenic cotton lines expressing three genes (Cry1Ac-Cry2Ab-EPSPS) for lepidopteran insect pests and herbicide tolerance.Scientific reports · 2022Article
- Development and performance evaluation of whole-genome sequencing with paired-end and mate-pair strategies in molecular characterization of GM crops: One GM rice 114-7-2 line as an example.Food chemistry. Molecular sciences · 2022Article
- A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice.Communications biology · 2022Article
- Whole-genome sequencing identifies I-SceI-mediated transgene integration sites in Xenopus tropicalis snai2:eGFP line.G3 (Bethesda, Md.) · 2022Article
- LIFE-Seq: a universal Large Integrated DNA Fragment Enrichment Sequencing strategy for deciphering the transgene integration of genetically modified organisms.Plant biotechnology journal · 2022Article
- TC-hunter: identification of the insertion site of a transgenic gene within the host genome.BMC genomics · 2022Article
- Generation ofMicroorganisms · 2019Article
- A novel glycosylated anti-CD20 monoclonal antibody from transgenic cattle.Scientific reports · 2018Article
- Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer.BMC genomics · 2018Article
- Application of droplet digital PCR in the analysis of genome integration and organization of the transgene in BAC transgenic mice.Scientific reports · 2018Article
- Genetic characteristics of polycistronic system‑mediated randomly‑inserted multi‑transgenes in miniature pigs and mice.Molecular medicine reports · 2018Article
- The increased expression of follicle-stimulating hormone leads to a decrease of fecundity in transgenic Large White female pigs.Transgenic research · 2017Article
- Genome-association analysis of Korean Holstein milk traits using genomic estimated breeding value.Asian-Australasian journal of animal sciences · 2017Article
- Application of whole genome shotgun sequencing for detection and characterization of genetically modified organisms and derived products.Analytical and bioanalytical chemistry · 2016Review
- Efficient generation of transgenic cattle using the DNA transposon and their analysis by next-generation sequencing.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As the number of transgenic livestock increases, reliable detection and molecular characterization of transgene integration sites and copy number are crucial not only for interpreting the relationship between the integration site and the specific phenotype but also for commercial and economic demands. However, the ability of conventional PCR techniques to detect incomplete and multiple integration events is limited, making it technically challenging to characterize transgenes. Next-generation sequencing has enabled cost-effective, routine and widespread high-throughput genomic analysis. Here, we demonstrate the use of next-generation sequencing to extensively characterize cattle harboring a 150-kb human lactoferrin transgene that was initially analyzed by chromosome walking without success. Using this approach, the sites upstream and downstream of the target gene integration site in the host genome were identified at the single nucleotide level. The sequencing result was verified by event-specific PCR for the integration sites and FISH for the chromosomal location. Sequencing depth analysis revealed that multiple copies of the incomplete target gene and the vector backbone were present in the host genome. Upon integration, complex recombination was also observed between the target gene and the vector backbone. These findings indicate that next-generation sequencing is a reliable and accurate approach for the molecular characterization of the transgene sequence, integration sites and copy number in transgenic species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.