ArticlePloS one2012
Flow sorting and molecular cytogenetic identification of individual chromosomes of Dasypyrum villosum L. (H. villosa) by a single DNA probe.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Population-scale gene expression analysis reveals the contribution of expression diversity to the modern wheat improvement.Nature communications · 2025Article
- Chromosome diversity in Dasypyrum villosum, an important genetic and trait resource for hexaploid wheat engineering.Annals of botany · 2023Article
- Laser Capture Microdissection: From Genomes to Chromosomes, from Complex Tissue to Single-Cell Analysis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Development and application of specific FISH probes for karyotyping Psathyrostachys huashanica chromosomes.BMC genomics · 2022Article
- Flow karyotyping of wheat-Frontiers in plant science · 2022Article
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars.Scientific reports · 2019Article
- Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting.The plant genome · 2019Article
- Article
- Flow sorting of C-genome chromosomes from wild relatives of wheat Aegilops markgrafii, Ae. triuncialis and Ae. cylindrica, and their molecular organization.Annals of botany · 2015Article
- Flow cytometric chromosome sorting from diploid progenitors of bread wheat, T. urartu, Ae. speltoides and Ae. tauschii.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
- Introgression of A- and B-genome of tetraploid triticale chromatin into tetraploid rye.Journal of applied genetics · 2013Article
- FISHIS: fluorescence in situ hybridization in suspension and chromosome flow sorting made easy.PloS one · 2013Article
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Authors and funding
5 authors.
Funding
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Abstract
Dasypyrum villosum (L.) Candargy (sin. Haynaldia villosa) is an annual wild diploid grass species (2n = 2x = 14; genome VV) belonging to the Poaceae family, which is considered to be an important source of biotic and abiotic stress resistance genes for wheat breeding. Enhanced characterization of D. villosum chromosomes can facilitate exploitation of its gene pool and its use in wheat breeding programs. Here we present the cytogenetic identification of D. villosum chromosomes on slide by fluorescent in situ hybridization (FISH), with the GAA simple sequence repeat (SSR) as a probe. We also describe the isolation and the flow cytometric analysis of D. villosum chromosomes in suspension, resulting in a distinguished flow karyotype. Chromosomes were flow sorted into three fractions, according their DNA content, one of which was composed of a single type of chromosome, namely 6 V, sorted with over 85% purity. Chromosome 6 V is known to carry genes to code for important resistance and seed storage characteristics, and its isolation represents a new source of genetic traits and specific markers useful for wheat improvement.
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