ArticlePloS one2012
The C. elegans rab family: identification, classification and toolkit construction.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 94 citations in OpenAlex.
- An RNAi screen of Rab GTPase genes in Caenorhabditis elegans reveals that morphogenesis has a higher demand than stem cell niche maintenance for rab-1 in the somatic cells of the reproductive system.G3 (Bethesda, Md.) · 2025Article
- RAB-10 cooperates with EHBP-1 to capture vesicular carriers during post-Golgi exocytic trafficking.The Journal of cell biology · 2025Article
- RABR-1, an atypical Rab-related GTPase, cell-nonautonomously restricts somatosensory dendrite branching.Genetics · 2024Article
- Germline stem cell integrity and quiescence are controlled by an AMPK-dependent neuronal trafficking pathway.PLoS genetics · 2023Article
- Therapeutic Targeting of Rab GTPases: Relevance for Alzheimer's Disease.Biomedicines · 2022Review
- Rab GTPases: The principal players in crafting the regulatory landscape of endosomal trafficking.Computational and structural biotechnology journal · 2022Review
- rab-27 acts in an intestinal pathway to inhibit axon regeneration in C. elegans.PLoS genetics · 2021Article
- Large Rab GTPases: Novel Membrane Trafficking Regulators with a Calcium Sensor and Functional Domains.International journal of molecular sciences · 2021Review
- Plant RABs: Role in Development and in Abiotic and Biotic Stress Responses.Current genomics · 2021Review
- Article
- The evolutionary landscape of the Rab family in chordates.Cellular and molecular life sciences : CMLS · 2019Article
- The small GTPase RAB-35 defines a third pathway that is required for the recognition and degradation of apoptotic cells.PLoS genetics · 2018Article
- The presynaptic machinery at the synapse of C. elegans.Invertebrate neuroscience : IN · 2018Review
- Article
- RAB-10-Dependent Membrane Transport Is Required for Dendrite Arborization.PLoS genetics · 2015Article
- C. elegans as a model for membrane traffic.WormBook : the online review of C. elegans biology · 2014Review
- Caenorhabditis elegans HOPS and CCZ-1 mediate trafficking to lysosome-related organelles independently of RAB-7 and SAND-1.Molecular biology of the cell · 2014Article
- Article
Corrections and comments
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Authors and funding
59 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rab monomeric GTPases regulate specific aspects of vesicle transport in eukaryotes including coat recruitment, uncoating, fission, motility, target selection and fusion. Moreover, individual Rab proteins function at specific sites within the cell, for example the ER, golgi and early endosome. Importantly, the localization and function of individual Rab subfamily members are often conserved underscoring the significant contributions that model organisms such as Caenorhabditis elegans can make towards a better understanding of human disease caused by Rab and vesicle trafficking malfunction. With this in mind, a bioinformatics approach was first taken to identify and classify the complete C. elegans Rab family placing individual Rabs into specific subfamilies based on molecular phylogenetics. For genes that were difficult to classify by sequence similarity alone, we did a comparative analysis of intron position among specific subfamilies from yeast to humans. This two-pronged approach allowed the classification of 30 out of 31 C. elegans Rab proteins identified here including Rab31/Rab50, a likely member of the last eukaryotic common ancestor (LECA). Second, a molecular toolset was created to facilitate research on biological processes that involve Rab proteins. Specifically, we used Gateway-compatible C. elegans ORFeome clones as starting material to create 44 full-length, sequence-verified, dominant-negative (DN) and constitutive active (CA) rab open reading frames (ORFs). Development of this toolset provided independent research projects for students enrolled in a research-based molecular techniques course at California State University, East Bay (CSUEB).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.