ArticlePloS one2012
Early-life gut bacteria associate with IL-4-, IL-10- and IFN-γ production at two years of age.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Metagenomic assessment of the bacterial breastfeeding microbiome in mature milk across lactation.Frontiers in pediatrics · 2023Article
- Gut microbiota of endangered Australian sea lion pups is unchanged by topical ivermectin treatment for endemic hookworm infection.Frontiers in microbiology · 2022Article
- Effect of the Microbiome on Intestinal Innate Immune Development in Early Life and the Potential Strategy of Early Intervention.Frontiers in immunology · 2022Review
- Altered stress responses in adults born by Caesarean section.Neurobiology of stress · 2022Article
- Review
- Combinations of self-reported rhinitis, conjunctivitis, and asthma predicts IgE sensitization in more than 25,000 Danes.Clinical and translational allergy · 2021Article
- The Role of Short-Chain Fatty Acids in the Interplay between a Very Low-Calorie Ketogenic Diet and the Infant Gut Microbiota and Its Therapeutic Implications for Reducing Asthma.International journal of molecular sciences · 2020Review
- The Gut Microbiota, Kynurenine Pathway, and Immune System Interaction in the Development of Brain Cancer.Frontiers in cell and developmental biology · 2020Review
- Epigenetics and Malaria Susceptibility/Protection: A Missing Piece of the Puzzle.Frontiers in immunology · 2018Review
- Bacteriological and Immunological Profiling of Meconium and Fecal Samples from Preterm Infants: A Two-Year Follow-Up Study.Nutrients · 2017Article
- Lactobacillus reuteri and Staphylococcus aureus differentially influence the generation of monocyte-derived dendritic cells and subsequent autologous T cell responses.Immunity, inflammation and disease · 2016Article
- Associations between EBV and CMV Seropositivity, Early Exposures, and Gut Microbiota in a Prospective Birth Cohort: A 10-Year Follow-up.Frontiers in pediatrics · 2016Article
- Probiotic Lactobacilli Modulate Staphylococcus aureus-Induced Activation of Conventional and Unconventional T cells and NK Cells.Frontiers in immunology · 2016Article
- Development of the infant intestinal microbiome: A bird's eye view of a complex process.Birth defects research. Part C, Embryo today : reviews · 2015Review
- The first 1000 cultured species of the human gastrointestinal microbiota.FEMS microbiology reviews · 2014Review
- Staphylococcus aureus Colonization: Modulation of Host Immune Response and Impact on Human Vaccine Design.Frontiers in immunology · 2014Review
- Regulation of Intestinal Immune Responses through TLR Activation: Implications for Pro- and Prebiotics.Frontiers in immunology · 2014Review
- Lactobacilli regulate Staphylococcus aureus 161:2-induced pro-inflammatory T-cell responses in vitro.PloS one · 2013Article
- The Infant Microbiome: Implications for Infant Health and Neurocognitive Development.Nursing researchReview
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial exposure early in life influences immune maturation and potentially also the development of immune-mediated disease. Here we studied early-life gut colonization in relation to cytokine responses at two years of age. Fecal samples were collected from infants during the first two months of life. DNA was extracted from the fecal samples and Bifidobacterium (B.) adolescentis, B. breve, B. bifidum, a group of lactobacilli (L. casei, L. paracasei and L. rhamnosus) as well as Staphylococcus (S.) aureus were detected with real time PCR. Peripheral mononuclear cells were stimulated with phytohaemagglutinin (PHA) and numbers of IL-4-, IL-10- and IFN-γ secreting cells were evaluated using ELISpot. We further stimulated peripheral blood mononuclear cells with bacterial supernatants in vitro and assessed the IL-4-, IL-10- and IFN-γ inducing capacity by flow cytometry and ELISA. Early S. aureus colonization associated with higher numbers of IL-4- (p = 0.022) and IL-10 (p = 0.016) producing cells at two years of age. In contrast to colonization with S. aureus alone, co-colonization with lactobacilli associated with suppression of IL-4- (p = 0.004), IL-10- (p = 0.004) and IFN-γ (p = 0.034) secreting cells. In vitro stimulations of mononuclear cells with bacterial supernatants supported a suppressive role of L. rhamnosus GG on S. aureus-induced cytokine responses. We demonstrate that the early gut colonization pattern associates with the PHA-induced cytokine profile at two years of age and our in vitro findings support that specific bacterial species influence the T helper cell subsets. This suggests that dysbiosis in the early microbiota may modulate the risk of developing inflammatory conditions like allergy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.