ArticleProceedings of the National Academy of Sciences of the United States of America2012
Late embryogenesis abundant proteins protect human hepatoma cells during acute desiccation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 117 citations in OpenAlex.
- Screening of Xeroprotectants for Efficient Mammalian Cell Structural Preservation in Dry Conditions.ACS omega · 2026Article
- Molecular timekeepers: the curious alliance of redox, repair, and protective proteins in preserving seed longevity.Crop health · 2026Review
- Genome-wide identification, structural characterization and gene expression analysis of the LEA_2 gene family in faba bean (Vicia Faba L.).BMC plant biology · 2025Article
- Life on the dry side: a roadmap to understanding desiccation tolerance and accelerating translational applications.Nature communications · 2025Review
- Tardigrade secretory proteins protect biological structures from desiccation.Communications biology · 2024Article
- Dynamic conformational changes of a tardigrade group-3 late embryogenesis abundant protein modulate membrane biophysical properties.PNAS nexus · 2024Article
- The molecular basis of cereal grain proteostasis.Essays in biochemistry · 2022Review
- Deciphering the Biological Enigma-Genomic Evolution Underlying Anhydrobiosis in the Phylum Tardigrada and the ChironomidInsects · 2022Review
- Long-term preservation of germ cells and gonadal tissues at ambient temperatures.Reproduction & fertility · 2022Review
- Natural and Designed Proteins Inspired by Extremotolerant Organisms Can Form Condensates and Attenuate Apoptosis in Human Cells.ACS synthetic biology · 2022Article
- Protection by desiccation-tolerance proteins probed at the residue level.Protein science : a publication of the Protein Society · 2022Article
- LEA motifs promote desiccation tolerance in vivo.BMC biology · 2021Article
- Article
- Cryopreservation of NK and T Cells Without DMSO for Adoptive Cell-Based Immunotherapy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- The genome of the extremophile Artemia provides insight into strategies to cope with extreme environments.BMC genomics · 2021Article
- Genome-wide association analysis reveals seed protein loci as determinants of variations in grain mold resistance in sorghum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Pleiotropic roles of late embryogenesis abundant proteins ofComputational and structural biotechnology journal · 2021Review
- Article
- Late Embryogenesis Abundant Protein-Client Protein Interactions.Plants (Basel, Switzerland) · 2020Review
- Common Functions of Disordered Proteins across Evolutionary Distant Organisms.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Expression of late embryogenesis abundant (LEA) proteins is highly correlated with desiccation tolerance in anhydrobiotic animals, selected land plants, and bacteria. Genes encoding two LEA proteins, one localized to the cytoplasm/nucleus (AfrLEA2) and one targeted to mitochondria (AfrLEA3m), were stably transfected into human HepG2 cells. A trehalose transporter was used for intracellular loading of this disaccharide. Cells were rapidly and uniformly desiccated to low water content (<0.12 g H(2)O/g dry weight) with a recently developed spin-drying technique. Immediately on rehydration, control cells without LEA proteins or trehalose exhibited 0% membrane integrity, compared with 98% in cells loaded with trehalose and expressing AfrLEA2 or AfrLEA3m; surprisingly, AfrLEA3m without trehalose conferred 94% protection. Cell proliferation across 7 d showed an 18-fold increase for cells dried with AfrLEA3m and trehalose, compared with 27-fold for nondried controls. LEA proteins dramatically enhance desiccation tolerance in mammalian cells and offer the opportunity for engineering biostability in the dried state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.