ArticleProceedings of the National Academy of Sciences of the United States of America2012
Microfluidic chambers for monitoring leukocyte trafficking and humanized nano-proresolving medicines interactions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.
- Cell and tissue engineering in lymph nodes for cancer immunotherapy.Advanced drug delivery reviews · 2020Pooled it
- Quantifying the Dual Effect of Antitumor and Pro-Tumor Human Neutrophils on Natural Killer Cell Behaviors in a Microphysiological System.ACS biomaterials science & engineering · 2026Article
- Microfluidic engineering of immune-competent organs-on-chips and their applications.Frontiers in immunology · 2026Review
- Microfluidic tools to model, monitor, and modulate the gut-brain axis.Biomicrofluidics · 2025Article
- Resolvin D2 restores monocyte anisocytosis and mediates a shift toward classical monocytes ex vivo in blood samples from patients after major burns.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Article
- Modelling the innate immune system in microphysiological systems.Lab on a chip · 2024Review
- A microphysiological system reveals neutrophil contact-dependent attenuation of pancreatic tumor progression by CXCR2 inhibition-based immunotherapy.Scientific reports · 2024Article
- Secretomes of M1 and M2 macrophages decrease the release of neutrophil extracellular traps.Scientific reports · 2023Article
- Passive redirection filters minimize red blood cell contamination during neutrophil chemotaxis assays using whole blood.Lab on a chip · 2023Article
- Modifiable risk factors linked to the development of rheumatoid arthritis: evidence, immunological mechanisms and prevention.Frontiers in immunology · 2023Review
- Lipoxin A4 attenuates the lung ischaemia reperfusion injury in rats after lung transplantation.Annals of medicine · 2021Article
- Regulation of Neutrophil Function by Marine n-3 Fatty Acids-A Mini Review.Cell biochemistry and biophysics · 2021Review
- Specialized Pro-Resolving Mediators as Potential Regulators of Inflammatory Macrophage Responses in COVID-19.Frontiers in immunology · 2021Review
- Critical Considerations for the Design of Multi-Organ Microphysiological Systems (MPS).Frontiers in cell and developmental biology · 2021Review
- Lymph Nodes-On-Chip: Promising Immune Platforms for Pharmacological and Toxicological Applications.Frontiers in pharmacology · 2021Review
- Electrotaxis-on-Chip to Quantify Neutrophil Migration Towards Electrochemical Gradients.Frontiers in immunology · 2021Article
- Combined Effects of Electric Stimulation and Microgrooves in Cardiac Tissue-on-a-Chip for Drug Screening.Small methods · 2020Article
- Immune Organs and Immune Cells on a Chip: An Overview of Biomedical Applications.Micromachines · 2020Review
- 3D Immunocompetent Organ-on-a-Chip Models.Small methods · 2020Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Leukocyte trafficking plays a critical role in determining the progress and resolution of inflammation. Although significant progress has been made in understanding the role of leukocyte activation in inflammation, dissecting the interactions between different leukocyte subpopulations during trafficking is hampered by the complexity of in vivo conditions and the lack of detail of current in vitro assays. To measure the effects of the interactions between neutrophils and monocytes migrating in response to various chemoattractants, at single-cell resolution, we developed a microfluidic platform that replicates critical features of focal inflammation sites. We integrated an elastase assay into the focal chemotactic chambers (FCCs) of our device that enabled us to distinguish between phlogistic and nonphlogistic cell recruitment. We found that lipoxin A(4) and resolvin D1, in solution or incorporated into nano-proresolving medicines, reduced neutrophil and monocyte trafficking toward leukotriene B(4). Lipoxin A(4) also reduced the elastase release from homogenous and heterogenous mixtures of neutrophils and monocytes. Surprisingly, the effect of resolvin D1 on heterogenous mixtures was antisynergistic, resulting in a transient spike in elastase activity, which was quickly terminated, and the degraded elastin removed by the leukocytes inside the FCCs. Therefore, the microfluidic assay provides a robust platform for measuring the effect of leukocyte interactions during trafficking and for characterizing the effects of inflammation mediators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.