ArticleProceedings of the National Academy of Sciences of the United States of America2012
Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 56 citations in OpenAlex.
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- Time-resolved RNA signatures of CD4+ T cells in Parkinson's disease.Cell death discovery · 2023Article
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- microRNA-132 inhibits the proliferation, migration, and invasion of ovarian cancer cells by regulating CT10 oncogenic gene homolog II-related signaling pathways.Translational cancer research · 2020Article
- Hypoxia-Induced MicroRNA-212/132 Alter Blood-Brain Barrier Integrity Through Inhibition of Tight Junction-Associated Proteins in Human and Mouse Brain Microvascular Endothelial Cells.Translational stroke research · 2019Article
- miR-103 promotes endothelial maladaptation by targeting lncWDR59.Nature communications · 2018Article
- Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs.Journal of visualized experiments : JoVE · 2018Article
- The Stress-Responding miR-132-3p Shows Evolutionarily Conserved Pathway Interactions.Cellular and molecular neurobiology · 2018Article
- Detection of dietetically absorbed maize-derived microRNAs in pigs.Scientific reports · 2017Article
- Dicer generates a regulatory microRNA network in smooth muscle cells that limits neointima formation during vascular repair.Cellular and molecular life sciences : CMLS · 2017Article
- MicroRNA regulation of endothelial TREX1 reprograms the tumour microenvironment.Nature communications · 2016Article
- microRNA-132/212 deficiency enhances Aβ production and senile plaque deposition in Alzheimer's disease triple transgenic mice.Scientific reports · 2016Article
- MiR-210 promotes sensory hair cell formation in the organ of corti.BMC genomics · 2016Article
- Endothelial Dicer promotes atherosclerosis and vascular inflammation by miRNA-103-mediated suppression of KLF4.Nature communications · 2016Article
- Cross-kingdom inhibition of breast cancer growth by plant miR159.Cell research · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Identifying targets is critical for understanding the biological effects of microRNA (miRNA) expression. The challenge lies in characterizing the cohort of targets for a specific miRNA, especially when targets are being actively down-regulated in miRNA- RNA-induced silencing complex (RISC)-messengerRNA (mRNA) complexes. We have developed a robust and versatile strategy called RISCtrap to stabilize and purify targets from this transient interaction. Its utility was demonstrated by determining specific high-confidence target datasets for miR-124, miR-132, and miR-181 that contained known and previously unknown transcripts. Two previously unknown miR-132 targets identified with RISCtrap, adaptor protein CT10 regulator of kinase 1 (CRK1) and tight junction-associated protein 1 (TJAP1), were shown to be endogenously regulated by miR-132 in adult mouse forebrain. The datasets, moreover, differed in the number of targets and in the types and frequency of microRNA recognition element (MRE) motifs, thus revealing a previously underappreciated level of specificity in the target sets regulated by individual miRNAs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.