ArticleProceedings of the National Academy of Sciences of the United States of America2012
Structural genes of wheat and barley 5-methylcytosine DNA glycosylases and their potential applications for human health.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 99 citations in OpenAlex.
- Pooled it
- Review
- Impact of a Gluten-Free Diet in Adults With Celiac Disease: Nutritional Deficiencies and Challenges.Cureus · 2024Review
- Prospects for developing allergen-depleted food crops.The plant genome · 2023Review
- Base Excision DNA Repair in Plants:International journal of molecular sciences · 2023Review
- Loss of Function of the RRMF Domain in OsROS1a Causes Sterility in Rice (International journal of molecular sciences · 2022Article
- Wheat genomic study for genetic improvement of traits in China.Science China. Life sciences · 2022Review
- The Function of DNA Demethylase Gene ROS1a Null Mutant on Seed Development in Rice (International journal of molecular sciences · 2022Article
- DNA demethylation affects imprinted gene expression in maize endosperm.Genome biology · 2022Article
- Promoter DNA hypermethylation ofJournal of advanced research · 2022Article
- Wheat Quality Formation and Its Regulatory Mechanism.Frontiers in plant science · 2022Review
- Targeting Induced Local Lesions in the WheatFrontiers in nutrition · 2022Article
- The Gluten-Free Diet for Celiac Disease and Beyond.Nutrients · 2021Review
- Proteome Analysis and Epitope Mapping in a Commercial Reduced-Gluten Wheat Product.Frontiers in nutrition · 2021Article
- Genomic and functional genomics analyses of gluten proteins and prospect for simultaneous improvement of end-use and health-related traits in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Multi-Locus GWAS of Quality Traits in Bread Wheat: Mining More Candidate Genes and Possible Regulatory Network.Frontiers in plant science · 2020Article
- Prolamin Content and Grain Weight in RNAi Silenced Wheat Lines Under Different Conditions of Temperature and Nitrogen Availability.Frontiers in plant science · 2020Article
- Directed-Mutagenesis ofFrontiers in nutrition · 2020Article
- Stimulatory Response of Celiac Disease Peripheral Blood Mononuclear Cells Induced by RNAi Wheat Lines Differing in Grain Protein Composition.Nutrients · 2019Article
- Gluten Detection Methods and Their Critical Role in Assuring Safe Diets for Celiac Patients.Nutrients · 2019Review
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 3 countries.
Funding
Abstract
Wheat supplies about 20% of the total food calories consumed worldwide and is a national staple in many countries. Besides being a key source of plant proteins, it is also a major cause of many diet-induced health issues, especially celiac disease. The only effective treatment for this disease is a total gluten-free diet. The present report describes an effort to develop a natural dietary therapy for this disorder by transcriptional suppression of wheat DEMETER (DME) homeologs using RNA interference. DME encodes a 5-methylcytosine DNA glycosylase responsible for transcriptional derepression of gliadins and low-molecular-weight glutenins (LMWgs) by active demethylation of their promoters in the wheat endosperm. Previous research has demonstrated these proteins to be the major source of immunogenic epitopes. In this research, barley and wheat DME genes were cloned and localized on the syntenous chromosomes. Nucleotide diversity among DME homeologs was studied and used for their virtual transcript profiling. Functional conservation of DME enzyme was confirmed by comparing the motif and domain structure within and across the plant kingdom. Presence and absence of CpG islands in prolamin gene sequences was studied as a hallmark of hypo- and hypermethylation, respectively. Finally the epigenetic influence of DME silencing on accumulation of LMWgs and gliadins was studied using 20 transformants expressing hairpin RNA in their endosperm. These transformants showed up to 85.6% suppression in DME transcript abundance and up to 76.4% reduction in the amount of immunogenic prolamins, demonstrating the possibility of developing wheat varieties compatible for the celiac patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.