ArticleThe Journal of biological chemistry2013
Mechanistic target of rapamycin complex 1 (mTORC1)-mediated phosphorylation is governed by competition between substrates for interaction with raptor.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Timing and distribution of protein ingestion during prolonged recovery from resistance exercise alters myofibrillar protein synthesis.The Journal of physiology · 2013Trial
- Role of PIM Kinase Inhibitor in the Treatment of Alzheimer's Disease.Molecular neurobiology · 2024Review
- Effects of different acute stressors on the regulation of appetite genes in the carp (Royal Society open science · 2023Article
- Mitochondrial genotype alters the impact of rapamycin on the transcriptional response to nutrients in Drosophila.BMC genomics · 2021Article
- mTOR signalling pathway - A root cause for idiopathic autism?BMB reports · 2019Review
- Article
- Direct imaging of the recruitment and phosphorylation of S6K1 in the mTORC1 pathway in living cells.Scientific reports · 2019Article
- Article
- mTOR Signaling in Parkinson's Disease.Neuromolecular medicine · 2017Review
- Article
- Article
- Retroviral vectors elevate coexpressed protein levels in trans through cap-dependent translation.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Vitamin C and E supplementation alters protein signalling after a strength training session, but not muscle growth during 10 weeks of training.The Journal of physiology · 2014Article
- Reduced REDD1 expression contributes to activation of mTORC1 following electrically induced muscle contraction.American journal of physiology. Endocrinology and metabolism · 2014Article
- REDD1 enhances protein phosphatase 2A-mediated dephosphorylation of Akt to repress mTORC1 signaling.Science signaling · 2014Article
- Targeting the mTOR signaling network for Alzheimer's disease therapy.Molecular neurobiology · 2014Review
- Regulated in DNA damage and development 1 (REDD1) promotes cell survival during serum deprivation by sustaining repression of signaling through the mechanistic target of rapamycin in complex 1 (mTORC1).Cellular signalling · 2013Article
- mTOR and regulation of energy homeostasis in humans.Journal of molecular medicine (Berlin, Germany) · 2013Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
In this study, the interaction of mTORC1 with its downstream targets p70S6K1 and 4E-BP1 was evaluated in both mouse liver and mouse embryonic fibroblasts following combined disruption of the genes encoding 4E-BP1 and 4E-BP2. Phosphorylation of p70S6K1 was dramatically elevated in the livers of mice lacking 4E-BP1 and 4E-BP2 following feeding-induced activation of mTORC1. Immunoprecipitation of mTORC1 suggested that elevated phosphorylation was the result of enhanced interaction of p70S6K1 with raptor. These findings were extended to a cell culture system wherein loss of 4E-BP1 and 4E-BP2 resulted in elevated interaction of p70S6K1 with IGF1-induced activation of mTORC1 in conjunction with an enhanced rate of p70S6K1 phosphorylation at Thr-389. Furthermore, cotransfecting HA-p70S6K1 with 4E-BP1, but not 4E-BP1(F114A), reduced recovery of mTORC1 in HA-p70S6K1 immunoprecipitates. Together, these findings support the conclusion that, in the absence of 4E-BP proteins, mTORC1-mediated phosphorylation of p70S6K1 is elevated by a reduction in competition between the two substrates for interaction with raptor.
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Registered trials
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