ArticleCurrent protocols in microbiology2012
Sphingolipid trafficking and purification in Chlamydia trachomatis-infected cells.
Article in Current protocols in microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- The Small Molecule H89 InhibitsInfection and immunity · 2021Article
- Sphingolipid Metabolism and Transport inFrontiers in cell and developmental biology · 2019Review
- Development of a Proximity Labeling System to Map theFrontiers in cellular and infection microbiology · 2017Article
- The trans-Golgi SNARE syntaxin 10 is required for optimal development of Chlamydia trachomatis.Frontiers in cellular and infection microbiology · 2015Article
- Vesicle-associated membrane protein 4 and syntaxin 6 interactions at the chlamydial inclusion.Infection and immunity · 2013Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
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Abstract
Chlamydia trachomatis is an obligate intracellular human pathogen, which lacks a system that allows genetic manipulation. Therefore, chlamydial researchers must manipulate the host cell to better understand chlamydial biology. Host-derived lipid acquisition is critical for chlamydial survival within the host. Hence, the ability to track and purify sphingolipids in/from chlamydial infected cells has become an integral part of pivotal studies in chlamydial biology. This unit outlines protocols that provide details about labeling eukaryotic cells with exogenous lipids to examine Golgi-derived lipid trafficking to the chlamydial inclusion and then performing imaging studies or lipid extractions for quantification. Details are provided to allow these protocols to be applied to subconfluent, polarized, or siRNA knockdown cells. In addition, one will find important experimental design considerations and techniques. These methods are powerful tools to aid in the understanding of mechanisms, which allow C. trachomatis to manipulate and usurp host cell trafficking pathways.
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Registered trials
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