ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2013
A technique to increase accessibility to late-stage chick embryos for in ovo manipulations.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Relatively Rare Populations of Invasive Cells Drive Progression of Heterogeneous Tumors.Cellular and molecular bioengineering · 2024Article
- Nociception in Chicken Embryos, Part III: Analysis of Movements before and after Application of a Noxious Stimulus.Animals : an open access journal from MDPI · 2023Article
- Nociception in Chicken Embryos, Part I: Analysis of Cardiovascular Responses to a Mechanical Noxious Stimulus.Animals : an open access journal from MDPI · 2023Article
- Towards human organ generation using interspecies blastocyst complementation: Challenges and perspectives for therapy.Frontiers in cell and developmental biology · 2023Review
- Article
- Periocular neural crest cell differentiation into corneal endothelium is influenced by signals in the nascent corneal environment.Developmental biology · 2020Article
- Recapitulation of normal collagen architecture in embryonic wounded corneas.Scientific reports · 2020Article
- The Chicken as a Model Organism to Study Heart Development.Cold Spring Harbor perspectives in biology · 2020Review
- Effect of In Ovo Injection of L-Arginine in Different Chicken Embryonic Development Stages on Post-Hatchability, Immune Response, and Myo-D and Myogenin Proteins.Animals : an open access journal from MDPI · 2019Article
- Optimized ex-ovo culturing of chick embryos to advanced stages of development.Journal of visualized experiments : JoVE · 2015Article
- Wounded embryonic corneas exhibit nonfibrotic regeneration and complete innervation.Investigative ophthalmology & visual science · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDuring early development, avian embryos are easily accessible in ovo for transplantations and experimental perturbations. However, these qualities of the avian embryonic model rapidly wane shortly after embryonic day (E)4 when the embryo is obscured by extraembryonic membranes, making it difficult to study developmental events that occur at later stages in vivo.
resultsIn this study, we describe a multistep method that involves initially windowing eggs at E3, followed by dissecting away extraembryonic membranes at E5 to facilitate embryo accessibility in ovo until later stages of development. The majority of the embryos subjected to this technique remain exposed between E5 and E8, then become gradually displaced by the growing allantois from posterior to anterior regions.
conclusionsExposed embryos are viable and compatible with embryological and modern developmental biology techniques including tissue grafting and ablation, gene manipulation by electroporation, and protein expression. This technique opens up new avenues for studying complex cellular interactions during organogenesis and can be further extrapolated to regeneration and stem cell studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.