ArticleJournal of biophotonics2013
Automated fluorescence lifetime imaging plate reader and its application to Förster resonant energy transfer readout of Gag protein aggregation.
Article in Journal of biophotonics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- High-throughput, multi-parametric, and correlative fluorescence lifetime imaging.Methods and applications in fluorescence · 2020Review
- Fast Fluorescence Lifetime Imaging Reveals the Aggregation Processes of α-Synuclein and Polyglutamine in AgingACS chemical biology · 2019Article
- Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells.SLAS technology · 2019Article
- Combining TIR and FRET in Molecular Test Systems.International journal of molecular sciences · 2019Article
- Mouse Stbd1 isJournal of cell science · 2017Article
- Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM).Scientific reports · 2016Article
- Intravital FRET: Probing Cellular and Tissue Function in Vivo.International journal of molecular sciences · 2015Article
- Fluorescence anisotropy (polarization): from drug screening to precision medicine.Expert opinion on drug discovery · 2015Review
- Global profiling of co- and post-translationally N-myristoylated proteomes in human cells.Nature communications · 2014Article
- Article
- Reduced temporal sampling effect on accuracy of time-domain fluorescence lifetime Förster resonance energy transfer.Journal of biomedical optics · 2014Article
- Developments in preclinical cancer imaging: innovating the discovery of therapeutics.Nature reviews. Cancer · 2014Review
- Automated selection of regions of interest for intensity-based FRET analysis of transferrin endocytic trafficking in normal vs. cancer cells.Methods (San Diego, Calif.) · 2014Article
- Active wide-field illumination for high-throughput fluorescence lifetime imaging.Optics letters · 2013Article
- Article
- FLIM-FRET for Cancer Applications.Current molecular imagingArticle
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Authors and funding
14 authors.
Funding
Abstract
Fluorescence lifetime measurements can provide quantitative readouts of local fluorophore environment and can be applied to biomolecular interactions via Förster resonant energy transfer (FRET). Fluorescence lifetime imaging (FLIM) can therefore provide a high content analysis (HCA) modality to map protein-protein interactions (PPIs) with applications in drug discovery, systems biology and basic research. We present here an automated multiwell plate reader able to perform rapid unsupervised optically sectioned FLIM of fixed and live biological samples and illustrate its potential to assay PPIs through application to Gag protein aggregation during the HIV life cycle. We demonstrate both hetero-FRET and homo-FRET readouts of protein aggregation and report the first quantitative evaluation of a FLIM HCA assay by generating dose response curves through addition of an inhibitor of Gag myristoylation. Z' factors exceeding 0.6 are realised for this FLIM FRET assay.
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