ArticleApplied and environmental microbiology2013
Metabolic engineering of Synechocystis sp. strain PCC 6803 for isobutanol production.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed, 178 citations in OpenAlex.
- Adaptive Laboratory Evolution inMicroorganisms · 2026Review
- Photosynthetic isobutanol production by integrating pathway engineering with carbon sink removal in cyanobacteria under outdoor natural sunlight.Bioprocess and biosystems engineering · 2025Article
- Directed evolution of α-ketoisovalerate decarboxylase for improved isobutanol and 3-methyl-1-butanol production in cyanobacteria.Biotechnology for biofuels and bioproducts · 2025Article
- Microbial host engineering for sustainable isobutanol production from renewable resources.Applied microbiology and biotechnology · 2024Review
- Review of recent advances in improvement strategies for biofuels production from cyanobacteria.Heliyon · 2024Article
- Techno-Economic Assessment of Electromicrobial Production ofEnvironmental science & technology · 2024Article
- Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803.Biotechnology for biofuels and bioproducts · 2023Article
- Unravelling the hidden power of esterases for biomanufacturing of short-chain esters.Scientific reports · 2023Article
- Metabolic Engineering Design Strategies for Increasing Carbon Fluxes Relevant for Biosynthesis in Cyanobacteria.Advances in biochemical engineering/biotechnology · 2023Article
- Proximity Labeling Facilitates Defining the Proteome Neighborhood of Photosystem II Oxygen Evolution Complex in a Model Cyanobacterium.Molecular & cellular proteomics : MCP · 2022Article
- Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels.Bioengineered · 2022Review
- Transcriptomics and metabolomics of engineered Synechococcus elongatus during photomixotrophic growth.Microbial cell factories · 2022Article
- Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria.Microbial cell factories · 2022Article
- Introduction of NADH-dependent nitrate assimilation in Synechococcus sp. PCC 7002 improves photosynthetic production of 2-methyl-1-butanol and isobutanol.Metabolic engineering · 2022Article
- Cyanobacteria as a Promising Alternative for Sustainable Environment: Synthesis of Biofuel and Biodegradable Plastics.Frontiers in microbiology · 2022Review
- Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria.Nucleic acids research · 2021Article
- Approaches in the photosynthetic production of sustainable fuels by cyanobacteria using tools of synthetic biology.World journal of microbiology & biotechnology · 2021Review
- Establishment of a resource recycling strategy by optimizing isobutanol production in engineered cyanobacteria using high salinity stress.Biotechnology for biofuels · 2021Article
- Algal glycobiotechnology: omics approaches for strain improvement.Microbial cell factories · 2021Review
- Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp. PCC 6803 in respect to expression efficiency and copy number.Microbial cell factories · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Global warming and decreasing fossil fuel reserves have prompted great interest in the synthesis of advanced biofuels from renewable resources. In an effort to address these concerns, we performed metabolic engineering of the cyanobacterium Synechocystis sp. strain PCC 6803 to develop a strain that can synthesize isobutanol under both autotrophic and mixotrophic conditions. With the expression of two heterologous genes from the Ehrlich pathway, the engineered strain can accumulate 90 mg/liter of isobutanol from 50 mM bicarbonate in a gas-tight shaking flask. The strain does not require any inducer (i.e., isopropyl β-d-1-thiogalactopyranoside [IPTG]) or antibiotics to maintain its isobutanol production. In the presence of glucose, isobutanol synthesis is only moderately promoted (titer = 114 mg/liter). Based on isotopomer analysis, we found that, compared to the wild-type strain, the mutant significantly reduced its glucose utilization and mainly employed autotrophic metabolism for biomass growth and isobutanol production. Since isobutanol is toxic to the cells and may also be degraded photochemically by hydroxyl radicals during the cultivation process, we employed in situ removal of the isobutanol using oleyl alcohol as a solvent trap. This resulted in a final net concentration of 298 mg/liter of isobutanol under mixotrophic culture conditions.
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