ArticleApplied and environmental microbiology2013
Inhibition of Staphylococcus aureus invasion into bovine mammary epithelial cells by contact with live Lactobacillus casei.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- Global spatiotemporal patterns of bovine mastitis pathogens from 2000 to 2025: a meta-analysis for precision dairy health.Journal of animal science and biotechnology · 2026Article
- Probiotics as Sustainable Alternatives to Mitigate Antibiotic Resistance in Bovine Mastitis Management.Probiotics and antimicrobial proteins · 2026Review
- Prevention and Treatment ofPathogens (Basel, Switzerland) · 2026Review
- Reduced Oxygen Condition Is Associated with Genome-Wide Expression Changes in Mastitis-LineageInternational journal of molecular sciences · 2026Article
- The current studies on the mechanisms of lactic acid bacteria as probiotics in the alleviate of bovine diseases: a review.Frontiers in cellular and infection microbiology · 2026Review
- Preparation and characterization of Lactobacillus rhamnosus, Lactobacillus casei, glycyrrhizin, vitamin-D3, and citric acid-loaded mucoadhesive pectin microparticles for the management of dental caries.BMC pharmacology & toxicology · 2025Article
- Protective effects of metabolites from lactic acid bacteria against infections of mastitis pathogen in bovine cells.Scientific reports · 2025Article
- Alternative approaches to antibiotics in the control of mastitis in dairy cows: a review.Veterinary research communications · 2025Review
- A study on antimicrobial activity of lysine-like peptoids for the development of new antimicrobials.Archives of microbiology · 2025Article
- High throughput transcriptomics analysis of ovine mammary epithelial cells stimulated with Staphylococcus aureus in vitro.PloS one · 2025Article
- The preventive effect of probioticFrontiers in veterinary science · 2025Article
- In vitro characterization of probiotic potential of Lactobacillus plantarum CM49 against selected cattle mastitogens.BMC microbiology · 2024Article
- Metabolites of non-aureus staphylococci affect the ability of Staphylococcus aureus to adhere to and internalize into bovine mammary epithelial cells.Veterinary research · 2023Article
- Gene expression and cell culture assays reveal cheese isolate Lactococcus lactis MC5 may influence the virulence of Staphylococcus aureus.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Alternatives to antibiotics for treatment of mastitis in dairy cows.Frontiers in veterinary science · 2023Review
- Lactobacilli, a Weapon to Counteract Pathogens through the Inhibition of Their Virulence Factors.Journal of bacteriology · 2022Review
- Immunomodulatory Effects of Probiotics: A Novel Preventive Approach for the Control of Bovine Mastitis.Microorganisms · 2022Review
- Factors Affecting Spontaneous Endocytosis and Survival of Probiotic Lactobacilli in Human Intestinal Epithelial Cells.Microorganisms · 2022Article
- Prospecting of potentially probiotic lactic acid bacteria from bovine mammary ecosystem: imminent partners from bacteriotherapy against bovine mastitis.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- Non-antibiotic strategies for prevention and treatment of internalizedFrontiers in microbiology · 2022Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus aureus is a major pathogen that is responsible for mastitis in dairy herds. S. aureus mastitis is difficult to treat and prone to recurrence despite antibiotic treatment. The ability of S. aureus to invade bovine mammary epithelial cells (bMEC) is evoked to explain this chronicity. One sustainable alternative to treat or prevent mastitis is the use of lactic acid bacteria (LAB) as mammary probiotics. In this study, we tested the ability of Lactobacillus casei strains to prevent invasion of bMEC by two S. aureus bovine strains, RF122 and Newbould305, which reproducibly induce acute and moderate mastitis, respectively. L. casei strains affected adhesion and/or internalization of S. aureus in a strain-dependent manner. Interestingly, L. casei CIRM-BIA 667 reduced S. aureus Newbould305 and RF122 internalization by 60 to 80%, and this inhibition was confirmed for two other L. casei strains, including one isolated from bovine teat canal. The protective effect occurred without affecting bMEC morphology and viability. Once internalized, the fate of S. aureus was not affected by L. casei. It should be noted that L. casei was internalized at a low rate but survived in bMEC cells with a better efficiency than that of S. aureus RF122. Inhibition of S. aureus adhesion was maintained with heat-killed L. casei, whereas contact between live L. casei and S. aureus or bMEC was required to prevent S. aureus internalization. This first study of the antagonism of LAB toward S. aureus in a mammary context opens avenues for the development of novel control strategies against this major pathogen.
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