ArticleCurrent microbiology2013
Putative and unique gene sequence utilization for the design of species specific probes as modeled by Lactobacillus plantarum.
Article in Current microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- Modulations in the offspring gut microbiome are refractory to postnatal synbiotic supplementation among juvenile primates.BMC microbiology · 2018Article
- Red pepper powder is a crucial factor that influences the ontogeny of Weissella cibaria during kimchi fermentation.Scientific reports · 2016Article
- The influence of red pepper powder on the density of Weissella koreensis during kimchi fermentation.Scientific reports · 2015Article
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Abstract
The concept of utilizing putative and unique gene sequences for the design of species specific probes was tested. The abundance profile of assigned functions within the Lactobacillus plantarum genome was used for the identification of the putative and unique gene sequence, csh. The targeted gene (csh) was used as the template for PCR amplification and construction of a non-radioactive DIG labeled probe. The csh derived probe aided in the preliminary and rapid identification of L. plantarum from mixed cultures by colony hybridization. The method described here for the rapid identification of L. plantarum can also be applied for the rapid detection of other bacteria if a unique gene sequence can be identified from its complete genome sequence.
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