Evidence map›Paper›PMID 23183324›Full record

Trial reportFree radical biology & medicine2013

Physiological role for nitrate-reducing oral bacteria in blood pressure control.

Vikas Kapil, Syed M A Haydar, Vanessa Pearl, Jon O Lundberg, Eddie Weitzberg, Amrita Ahluwalia

2 registry-linked trialsOpen access · hybridAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Free radical biology & medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 182 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
182citing papers in PubMed, 6 pooled it
9.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03068962 naunknown statusnot on this mapstarted 2017, after this paper: background citation

Examination of the Distribution of Nitrate Reducing Bacteria in the Human Oral Cavity

TypeinterventionalSponsorUniversity of ReadingRan2017 to 2018Enrolled20ConditionsNo Dental Disease, Non-smokingArmsbeetroot Juice, Buxton water, antiseptic mouthwash then beetroot juice
NCT06375694 naunknown statusnot on this mapstarted 2024, after this paper: background citation

Oral Probiotic Effect on Dietary Nitrate to Plasma Nitrite Production

TypeinterventionalSponsorWake Forest UniversityRan2024 to 2025Enrolled20ConditionsHypertension, Cardiovascular Diseases, DiabetesArmsHerbiotics Oral + Ent Probiotic, Placebo
3 · Its place in the literature

Who cites it

182 citing papers in PubMed, 6 syntheses or guidelines pooled it, 395 citations in OpenAlex.

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122 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Vikas KapilCentre for Clinical Pharmacology, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London EC1M 6BQ, UK.
Syed M A Haydar
Vanessa Pearl
Jon O Lundberg
Eddie Weitzberg
Amrita Ahluwalia
Karolinska Institutet · SEQueen Mary University of London · GBWilliam Harvey Research Institute · GB

Funding

British Heart Foundation SP/08/006/25110
6 · The paper itself

Abstract

Circulating nitrate (NO(3)(-)), derived from dietary sources or endogenous nitric oxide production, is extracted from blood by the salivary glands, accumulates in saliva, and is then reduced to nitrite (NO(2)(-)) by the oral microflora. This process has historically been viewed as harmful, because nitrite can promote formation of potentially carcinogenic N-nitrosamines. More recent research, however, suggests that nitrite can also serve as a precursor for systemic generation of vasodilatory nitric oxide, and exogenous administration of nitrate reduces blood pressure in humans. However, whether oral nitrate-reducing bacteria participate in "setting" blood pressure is unknown. We investigated whether suppression of the oral microflora affects systemic nitrite levels and hence blood pressure in healthy individuals. We measured blood pressure (clinic, home, and 24-h ambulatory) in 19 healthy volunteers during an initial 7-day control period followed by a 7-day treatment period with a chlorhexidine-based antiseptic mouthwash. Oral nitrate-reducing capacity and nitrite levels were measured after each study period. Antiseptic mouthwash treatment reduced oral nitrite production by 90% (p < 0.001) and plasma nitrite levels by 25% (p = 0.001) compared to the control period. Systolic and diastolic blood pressure increased by 2-3 .5mmHg, increases correlated to a decrease in circulating nitrite concentrations (r(2) = 0.56, p = 0.002). The blood pressure effect appeared within 1 day of disruption of the oral microflora and was sustained during the 7-day mouthwash intervention. These results suggest that the recycling of endogenous nitrate by oral bacteria plays an important role in determination of plasma nitrite levels and thereby in the physiological control of blood pressure.

Indexed as

Blood PressureAdolescentAdultBacteriaBlood Pressure DeterminationCross-Over StudiesHumansMiddle AgedMouthNitratesNitritesReference ValuesYoung AdultNitratesNitrites

Identifiers

PMID23183324
PMCPMC3605573
OpenAlexW2087384948

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.