ArticleEndocrinology2013
Distribution and posttranslational modification of synaptic ERα in the adult female rat hippocampus.
Article in Endocrinology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 41 citations in OpenAlex.
- Female-specific dysfunction of sensory neocortical circuits in a mouse model of autism mediated by mGluR5 and estrogen receptor α.Cell reports · 2024Article
- Increased palmitoylation improves estrogen receptor alpha-dependent hippocampal synaptic deficits in a mouse model of synucleinopathy.Science advances · 2023Article
- Review
- Spatial and temporal specificity of neuroestradiol provision in the songbird.Journal of neuroendocrinology · 2023Review
- Hypomorphism of a Novel Long ERα Isoform Causes Severe Reproductive Dysfunctions in Female Mice.Endocrinology · 2022Article
- Estrogen receptor alpha, G-protein coupled estrogen receptor 1, and aromatase: Developmental, sex, and region-specific differences across the rat caudate-putamen, nucleus accumbens core and shell.The Journal of comparative neurology · 2021Article
- Endocrine disruption through membrane estrogen receptors and novel pathways leading to rapid toxicological and epigenetic effects.The Journal of steroid biochemistry and molecular biology · 2019Review
- The expression of select genes necessary for membrane-associated estrogen receptor signaling differ by sex in adult rat hippocampus.Steroids · 2019Article
- Neurosteroid Actions in Memory and Neurologic/Neuropsychiatric Disorders.Frontiers in endocrinology · 2019Review
- Palmitoylation of caveolin-1 is regulated by the same DHHC acyltransferases that modify steroid hormone receptors.The Journal of biological chemistry · 2018Article
- Interactions between estrogen receptors and metabotropic glutamate receptors and their impact on drug addiction in females.Hormones and behavior · 2018Review
- Membrane estrogen receptor signaling impacts the reward circuitry of the female brain to influence motivated behaviors.Steroids · 2018Review
- Experience-dependent homeostasis of 'noise' at inhibitory synapses preserves information coding in adult visual cortex.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
- Article
- Sex steroid hormones matter for learning and memory: estrogenic regulation of hippocampal function in male and female rodents.Learning & memory (Cold Spring Harbor, N.Y.) · 2015Review
- Sex Differences in Molecular Signaling at Inhibitory Synapses in the Hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Neurohormones, Brain, and Behavior: A Comparative Approach to Understanding Rapid Neuroendocrine Action.Integrative and comparative biology · 2015Article
- Estrogen receptors in the central nervous system and their implication for dopamine-dependent cognition in females.Hormones and behavior · 2015Review
- Opioid receptor-dependent sex differences in synaptic plasticity in the hippocampal mossy fiber pathway of the adult rat.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Membrane-initiated non-genomic signaling by estrogens in the hypothalamus: cross-talk with glucocorticoids with implications for behavior.Frontiers in endocrinology · 2015Review
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Acute 17β-estradiol (E2) signaling in the brain is mediated by extranuclear estrogen receptors. Here we used biochemical methods to investigate the distribution, posttranslational modification, and E2 regulation of estrogen receptor-α (ERα) in synaptosomal fractions isolated by differential centrifugation from the adult female rat hippocampus. We find that ERα is concentrated presynaptically and is highly enriched with synaptic vesicles. Immunoisolation of vesicles using vesicle subtype-specific markers showed that ERα is associated with both glutamate and γ-aminobutyric acid-containing neurotransmitter vesicles as well as with some large dense core vesicles. Experiments using broad spectrum and residue-specific phosphatases indicated that a portion of ERα in synaptosomal fractions is phosphorylated at serine/threonine residues leading to a mobility shift in SDS-PAGE and creating a double band on Western blots. The phosphorylated form of ERα runs in the upper of the two bands and is particularly concentrated with synaptic vesicles. Finally, we used E2 with or without the acyl protein thioesterase 1 inhibitor, Palmostatin B, to show that 20 min of E2 treatment of hippocampal slices depletes ERα from the synaptosomal membrane by depalmitoylation. We found no evidence that E2 regulates phosphorylation of synaptosomal ERα on this time scale. These studies begin to fill the gap between detailed molecular characterization of extranuclear ERα in previous in vitro studies and acute E2 modulation of hippocampal synapses in the adult brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.