ReviewDiagnostic microbiology and infectious disease2013
Viral diagnostics in the era of digital polymerase chain reaction.
Review in Diagnostic microbiology and infectious disease, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed.
- Droplet digital PCR-based pathogen monitoring system improves identifying Haemaphysalis longicornis infected with Bandavirus dabieense and other tick-borne pathogens at low concentrations.Parasites & vectors · 2026Article
- Molecular Differentiation of Intact West Nile Virus Using a PMAxx™-Enabled Digital PCR Workflow.International journal of molecular sciences · 2026Article
- Molecular insights into antimicrobial resistance in human bacterial pathogens: mechanisms, resistance genes, and translational diagnostic applications.Frontiers in microbiology · 2026Review
- Duplex digital PCR assay on microfluidic chamber arrays for total HIV DNA reservoir quantification in persons with HIV.Scientific reports · 2025Article
- Pico-Scale Digital PCR on a Super-Hydrophilic Microarray Chip for Multi-Target Detection.Micromachines · 2025Article
- Developing centrifugal force real-time digital PCR for detecting extremely low DNA concentration.Scientific reports · 2024Article
- Development of a triplex crystal digital RT-PCR for the detection of PHEV, PRV, and CSFV.Frontiers in veterinary science · 2024Article
- Detection and Quantification of Klebsiella pneumoniae in Fecal Samples Using Digital Droplet PCR in Comparison with Real-Time PCR.Microbiology spectrum · 2023Article
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- Article
- Detection of A and B Influenza Viruses by Surface-Enhanced Raman Scattering Spectroscopy and Machine Learning.Biosensors · 2022Article
- An alternative approach for bioanalytical assay optimization for wastewater-based epidemiology of SARS-CoV-2.The Science of the total environment · 2021Article
- Handheld plasmonic biosensor for virus detection in field-settings.Sensors and actuators. B, Chemical · 2021Article
- Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology.Current issues in molecular biology · 2021Review
- Development and assessment of a duplex droplet digital PCR method for quantification of GM rice Kemingdao.Analytical and bioanalytical chemistry · 2021Article
- Nucleic Acid Testing of SARS-CoV-2.International journal of molecular sciences · 2021Review
- Quantification and Trends of Rotavirus and Enterovirus in Untreated Sewage Using Reverse Transcription Droplet Digital PCR.Food and environmental virology · 2021Article
- Digital Droplet PCR for SARS-CoV-2 Resolves Borderline Cases.American journal of clinical pathology · 2021Article
- Triple Negative Breast Cancer: A Review of Present and Future Diagnostic Modalities.Medicina (Kaunas, Lithuania) · 2021Review
- Detecting Pathogen Exposure During the Non-symptomatic Incubation Period Using Physiological Data: Proof of Concept in Non-human Primates.Frontiers in physiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Unlike quantitative polymerase chain reaction (qPCR), digital PCR (dPCR) achieves sensitive and accurate absolute quantitation of a DNA sample without the need for a standard curve. A single PCR reaction is divided into many separate reactions that each have a positive or negative signal. By applying Poisson statistics, the number of DNA molecules in the original sample is directly calculated from the number of positive and negative reactions. The recent availability of multiple commercial dPCR platforms has led to increased interest in clinical diagnostic applications, such as low viral load detection and low abundance mutant detection, where dPCR could be superior to traditional qPCR. Here we review current literature that demonstrates dPCR's potential utility in viral diagnostics, particularly through absolute quantification of target DNA sequences and rare mutant allele detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.