ArticleBMC genomics2012
Comparative genomics and transcriptomics of trait-gene association.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
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- In vitro propagation and genome sequencing of three 'atypical' Ehrlichia ruminantium isolates.The Onderstepoort journal of veterinary research · 2020Article
- Macromolecular crowding links ribosomal protein gene dosage to growth rate in Vibrio cholerae.BMC biology · 2020Article
- Dynamics of repeat-associated plasticity in theGene: X · 2019Article
- Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns.Scientific reports · 2018Article
- Transcriptomic Determinants of Scrapie Prion Propagation in Cultured Ovine Microglia.PloS one · 2016Article
- Reduced Infectivity in cattle for an outer membrane protein mutant of Anaplasma marginale.Applied and environmental microbiology · 2015Article
- Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector.Parasites & vectors · 2014Article
- Knockout of an outer membrane protein operon of Anaplasma marginale by transposon mutagenesis.BMC genomics · 2014Article
- Genetic Diversity of Tick-Borne Rickettsial Pathogens; Insights Gained from Distant Strains.Pathogens (Basel, Switzerland) · 2014Article
- Whole-genome sequencing in bacteriology: state of the art.Infection and drug resistance · 2013Review
- Transcriptional pathways associated with the slow growth phenotype of transformed Anaplasma marginale.BMC genomics · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe Order Rickettsiales includes important tick-borne pathogens, from Rickettsia rickettsii, which causes Rocky Mountain spotted fever, to Anaplasma marginale, the most prevalent vector-borne pathogen of cattle. Although most pathogens in this Order are transmitted by arthropod vectors, little is known about the microbial determinants of transmission. A. marginale provides unique tools for studying the determinants of transmission, with multiple strain sequences available that display distinct and reproducible transmission phenotypes. The closed core A. marginale genome suggests that any phenotypic differences are due to single nucleotide polymorphisms (SNPs). We combined DNA/RNA comparative genomic approaches using strains with different tick transmission phenotypes and identified genes that segregate with transmissibility.
resultsComparison of seven strains with different transmission phenotypes generated a list of SNPs affecting 18 genes and nine promoters. Transcriptional analysis found two candidate genes downstream from promoter SNPs that were differentially transcribed. To corroborate the comparative genomics approach we used three RNA-seq platforms to analyze the transcriptomes from two A. marginale strains with different transmission phenotypes. RNA-seq analysis confirmed the comparative genomics data and found 10 additional genes whose transcription between strains with distinct transmission efficiencies was significantly different. Six regions of the genome that contained no annotation were found to be transcriptionally active, and two of these newly identified transcripts were differentially transcribed.
conclusionsThis approach identified 30 genes and two novel transcripts potentially involved in tick transmission. We describe the transcriptome of an obligate intracellular bacterium in depth, while employing massive parallel sequencing to dissect an important trait in bacterial pathogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.