ArticleACS nano2012
Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
Article in ACS nano, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 70 citations in OpenAlex.
- Weaker neuroligin 2-neurexin β1 interaction tethers membranes and recruits gephyrin at membrane junctions through clustering.Science advances · 2026Article
- Click biology highlights the opportunities from reliable biological reactions.Nature chemical biology · 2025Review
- Lipoic acid attachment to proteins: stimulating new developments.Microbiology and molecular biology reviews : MMBR · 2024Review
- Article
- Recent biomedical advances enabled by HaloTag technology.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2022Article
- Further assessments of ligase LplA-mediated modifications of proteins in vitro and in cellulo.Molecular biology reports · 2022Article
- Engineering a Metathesis-Catalyzing Artificial Metalloenzyme Based on HaloTag.ACS catalysis · 2021Article
- Engineering of a Red Fluorogenic Protein/Merocyanine Complex for Live-Cell Imaging.Chembiochem : a European journal of chemical biology · 2020Article
- Addition of Isocyanide-Containing Amino Acids to the Genetic Code for Protein Labeling and Activation.ACS chemical biology · 2019Article
- Quantum dots in biomedical applications.Acta biomaterialia · 2019Review
- Sensing with photoluminescent semiconductor quantum dots.Methods and applications in fluorescence · 2019Article
- New Routes and Opportunities for Modular Construction of Particulate Vaccines: Stick, Click, and Glue.Frontiers in immunology · 2018Review
- Live-cell single-molecule labeling and analysis of myosin motors with quantum dots.Molecular biology of the cell · 2017Article
- Nanoscale organization of synaptic adhesion proteins revealed by single-molecule localization microscopy.Neurophotonics · 2016Article
- Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin.Nature communications · 2016Article
- HaloTag technology: a versatile platform for biomedical applications.Bioconjugate chemistry · 2015Review
- Advances in chemical labeling of proteins in living cells.Cell and tissue research · 2015Review
- Multiplexed modular genetic targeting of quantum dots.ACS nano · 2014Article
- Computational design of a red fluorophore ligase for site-specific protein labeling in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Superglue from bacteria: unbreakable bridges for protein nanotechnology.Trends in biotechnology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second step, quantum dots derivatized with HaloTag, a modified haloalkane dehalogenase, react with the ligated bromodecanoic acid to form a covalent adduct. We found this targeting method to be specific, fast, and fully orthogonal to a previously reported and analogous quantum dot targeting method using E. coli biotin ligase and streptavidin. We used these two methods in combination for two-color quantum dot visualization of different proteins expressed on the same cell or on neighboring cells. Both methods were also used to track single molecules of neurexin, a synaptic adhesion protein, to measure its lateral diffusion in the presence of neuroligin, its trans-synaptic adhesion partner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.