ArticleJournal of proteome research2013
Novel phosphorylation sites in the S. cerevisiae Cdc13 protein reveal new targets for telomere length regulation.
Article in Journal of proteome research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA.Nucleic acids research · 2024Article
- Telomeres and Cancer.Life (Basel, Switzerland) · 2021Review
- Plant Growth and Drought Tolerance-Promoting Bacterium for Bioremediation of Paraquat Pesticide Residues in Agriculture Soils.Frontiers in microbiology · 2021Article
- Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- The mechanisms of K. lactis Cdc13 in telomere DNA-binding and telomerase regulation.DNA repair · 2018Article
- Regulation of telomere metabolism by the RNA processing protein Xrn1.Nucleic acids research · 2017Article
- Structure and function of the telomeric CST complex.Computational and structural biotechnology journal · 2016Review
- Identification of nuclear hormone receptor pathways causing insulin resistance by transcriptional and epigenomic analysis.Nature cell biology · 2015Article
- High resolution is not a strict requirement for characterization and quantification of histone post-translational modifications.Journal of proteome research · 2014Article
- Quantitative proteomic discovery of dynamic epigenome changes that control human cytomegalovirus (HCMV) infection.Molecular & cellular proteomics : MCP · 2014Article
- A quantitative atlas of histone modification signatures from human cancer cells.Epigenetics & chromatin · 2013Article
- DNA repair at telomeres: keeping the ends intact.Cold Spring Harbor perspectives in biology · 2013Review
- Quantitative dynamics of the link between cellular metabolism and histone acetylation.The Journal of biological chemistry · 2013Article
- Article
- Cdc13 at a crossroads of telomerase action.Frontiers in oncology · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The S. cerevisiae Cdc13 is a multifunctional protein with key roles in regulation of telomerase, telomere end protection, and conventional telomere replication, all of which are cell cycle-regulated processes. Given that phosphorylation is a key mechanism for regulating protein function, we identified sites of phosphorylation using nano liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS). We also determined phosphorylation abundance on both wild type (WT) and a telomerase deficient form of Cdc13, encoded by the cdc13-2 allele, in both G1 phase cells, when telomerase is not active, and G2/M phase cells, when it is. We identified 21 sites of in vivo phosphorylation, of which only five had been reported previously. In contrast, phosphorylation of two in vitro targets of the ATM-like Tel1 kinase, S249 and S255, was not detected. This result helps resolve conflicting data on the importance of phosphorylation of these residues in telomerase recruitment. Multiple residues showed differences in their cell cycle pattern of modification. For example, phosphorylation of S314 was significantly higher in the G2/M compared to the G1 phase and in WT versus mutant Cdc13, and a S314D mutation negatively affected telomere length. Our findings provide new targets in a key telomerase regulatory protein for modulation of telomere dynamics.
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