ArticleMacromolecules2012
Best practices for purification and characterization of PAMAM dendrimer.
Article in Macromolecules, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Transport of Anions across the Dialytic Membrane Induced by Complexation toward Dendritic Receptors.ACS omega · 2021Article
- Hybrid Nanosystems for Biomedical Applications.ACS nano · 2021Review
- Sulfonimide-Based Dendrimers: Progress in Synthesis, Characterization, and Potential Applications.Polymers · 2020Review
- Cartilage-penetrating nanocarriers improve delivery and efficacy of growth factor treatment of osteoarthritis.Science translational medicine · 2018Article
- Distributions: The Importance of the Chemist's Molecular View for Biological Materials.Biomacromolecules · 2018Review
- Increase in Dye:Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells.ACS biomaterials science & engineering · 2016Article
- Dendrimer antibody conjugate to target and image HER-2 overexpressing cancer cells.Oncotarget · 2016Article
- Rapid Exchange Between Free and Bound States in RNA-Dendrimer Polyplexes: Implications on the Mechanism of Delivery and Release.Biomacromolecules · 2016Article
- Generation 3 PAMAM dendrimer TAMRA conjugates containing precise dye/dendrimer ratios.Materials today (Kidlington, England) · 2015Article
- Quantitative Measurement of Cationic Polymer Vector and Polymer-pDNA Polyplex Intercalation into the Cell Plasma Membrane.ACS nano · 2015Article
- Preparation and long-term biodistribution studies of a PAMAM dendrimer G5-Gd-BnDOTA conjugate for lymphatic imaging.Nanomedicine (London, England) · 2015Article
- Multivalent polymers for drug delivery and imaging: the challenges of conjugation.Biomacromolecules · 2014Review
- Cationic PAMAM dendrimers as pore-blocking binary toxin inhibitors.Biomacromolecules · 2014Article
- Diffusion NMR study of generation-five PAMAM dendrimer materials.The journal of physical chemistry. B · 2014Article
- PAMAM Dendrimers as Quantized Building Blocks for Novel Nanostructures.Soft matter · 2013Article
- Quantitative analysis of generation and branch defects in G5 poly(amidoamine) dendrimer.Polymer · 2013Article
- Paclitaxel-conjugated PAMAM dendrimers adversely affect microtubule structure through two independent modes of action.Biomacromolecules · 2013Article
- Dendrimer nanoscaffolds for potential theranostics of prostate cancer with a focus on radiochemistry.Molecular pharmaceutics · 2013Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Poly(amidoamine) (PAMAM) dendrimer are branched polymers with low degrees of heterogeneity. Current synthesis methods, however, result in substantial batch variability. We present our optimized procedure for post-synthesis (and post-market) purification of a generation 5 PAMAM dendrimer by membrane dialysis and demonstrate its effectiveness and limitations using a representative lot of biomedical grade dendrimer. This method successfully removes trailing generation defect structures, thereby reducing the heterogeneity of the material (PDI reduced from 1.04 to 1.02). Optimized analytical techniques to characterize the unpurified and purified dendrimer are also detailed. The efficiency of the purification method is successfully monitored by these analytics and dendrimer parameters that are critical for subsequent modification reactions and biological evaluation (M(n), M(w), PDI, average number of end groups) obtained. To provide better definition of the variability that should be expected between lots of synthesized material, HPLC traces for three additional commercial lots of dendrimer are also presented.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.