ArticleNucleic acids research2013
Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- EPOP restricts PRC2.1 targeting to chromatin by directly modulating enzyme complex dimerization.Nature communications · 2026Article
- Cracking the Code of Neuronal Cell Fate.Cells · 2023Review
- Glutamatergic Neurons Differentiated from Embryonic Stem Cells: An Investigation of Differentiation and Associated Diseases.International journal of molecular sciences · 2021Review
- Dynamic Competition of Polycomb and Trithorax in Transcriptional Programming.Annual review of biochemistry · 2020Review
- A transcriptomic study of Williams-Beuren syndrome associated genes in mouse embryonic stem cells.Scientific data · 2019Article
- The gene regulatory network of mESC differentiation: a benchmark for reverse engineering methods.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions.Molecular systems biology · 2018Article
- EPOP Interacts with Elongin BC and USP7 to Modulate the Chromatin Landscape.Molecular cell · 2016Article
- Co-existence of intact stemness and priming of neural differentiation programs in mES cells lacking Trim71.Scientific reports · 2015Article
- The PRC2-associated factor C17orf96 is a novel CpG island regulator in mouse ES cells.Cell discovery · 2015Article
- Broad integration of expression maps and co-expression networks compassing novel gene functions in the brain.Scientific reports · 2014Article
- Regression hidden Markov modeling reveals heterogeneous gene expression regulation: a case study in mouse embryonic stem cells.BMC genomics · 2014Article
- Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 revealed by BioTAP-XL cross-linking and affinity purification.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Targeting molecular networks for drug research.Frontiers in genetics · 2014Review
- ESCAPE: database for integrating high-content published data collected from human and mouse embryonic stem cells.Database : the journal of biological databases and curation · 2013Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Gene expression profiles can be used to infer previously unknown transcriptional regulatory interaction among thousands of genes, via systems biology 'reverse engineering' approaches. We 'reverse engineered' an embryonic stem (ES)-specific transcriptional network from 171 gene expression profiles, measured in ES cells, to identify master regulators of gene expression ('hubs'). We discovered that E130012A19Rik (E13), highly expressed in mouse ES cells as compared with differentiated cells, was a central 'hub' of the network. We demonstrated that E13 is a protein-coding gene implicated in regulating the commitment towards the different neuronal subtypes and glia cells. The overexpression and knock-down of E13 in ES cell lines, undergoing differentiation into neurons and glia cells, caused a strong up-regulation of the glutamatergic neurons marker Vglut2 and a strong down-regulation of the GABAergic neurons marker GAD65 and of the radial glia marker Blbp. We confirmed E13 expression in the cerebral cortex of adult mice and during development. By immuno-based affinity purification, we characterized protein partners of E13, involved in the Polycomb complex. Our results suggest a role of E13 in regulating the division between glutamatergic projection neurons and GABAergic interneurons and glia cells possibly by epigenetic-mediated transcriptional regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.