ArticleNucleic acids research2013
The nucleoid-associated protein Dan organizes chromosomal DNA through rigid nucleoprotein filament formation in E. coli during anoxia.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy.Nature biomedical engineering · 2022Article
- RegulonDB 11.0: Comprehensive high-throughput datasets on transcriptional regulation inMicrobial genomics · 2022Article
- White spot syndrome viral protein VP9 alters the cellular higher-order chromatin structure.FASEB bioAdvances · 2020Article
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- Transfer-matrix calculations of the effects of tension and torque constraints on DNA-protein interactions.Nucleic acids research · 2018Article
- Role of Salt Valency in the Switch of H-NS Proteins between DNA-Bridging and DNA-Stiffening Modes.Biophysical journal · 2018Article
- Uncharacterized Bacterial Structures Revealed by Electron Cryotomography.Journal of bacteriology · 2017Article
- Morphology of the archaellar motor and associated cytoplasmic cone inEMBO reports · 2017Article
- Strategies for manipulation of oxygen utilization by the electron transfer chain in microbes for metabolic engineering purposes.Journal of industrial microbiology & biotechnology · 2017Review
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- Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF.Scientific reports · 2015Article
- Collaborative protein filaments.The EMBO journal · 2015Review
- Transcriptional Repressor TrmBL2 from Thermococcus kodakarensis Forms Filamentous Nucleoprotein Structures and Competes with Histones for DNA Binding in a Salt- and DNA Supercoiling-dependent Manner.The Journal of biological chemistry · 2015Article
- Mechanochemical regulations of RPA's binding to ssDNA.Scientific reports · 2015Article
- Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties.Nucleic acids research · 2014Article
- Mechanism of DNA organization by Mycobacterium tuberculosis protein Lsr2.Nucleic acids research · 2013Article
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5 authors.
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Abstract
Dan is a transcription factor that regulates the ttd operon encoding tartrate dehydratase. During anaerobic conditions, its copy number increases by 100-fold, making Dan an abundant nucleoid-associated protein. However, little is known about the mode of Dan-DNA interaction. To understand its cellular functions, we used single-molecule manipulation and imaging techniques to show that Dan binds cooperatively along DNA, resulting in formation of a rigid periodic nucleoprotein filament that strongly restricts accessibility to DNA. Furthermore, in the presence of physiologic levels of magnesium, these filaments interact with each other to cause global DNA condensation. Overall, these results shed light on the architectural role of Dan in the compaction of Escherichia coli chromosomal DNA under anaerobic conditions. Formation of the nucleoprotein filament provides a basis in understanding how Dan may play roles in both chromosomal DNA protection and gene regulation.
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