ArticleWorld journal of microbiology & biotechnology2013
Purification, characterization and kinetic properties of extracellular L-asparaginase produced by Cladosporium sp.
Article in World journal of microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 101 citations in OpenAlex.
- Novel glutaminase-free l-asparaginase fromRSC advances · 2026Article
- Biochemical characterisation of a new thermotolerant L-asparaginase produced by Cunninghamella echinulata PA3S12MM isolated from the Atlantic Forest.Archives of microbiology · 2026Article
- Pleurotus ostreatus L-asparaginase's use in food safety and biotechnology: from processing assistance to bioactive agent.Microbial cell factories · 2026Article
- Green bioprocessing of L-Asparaginase: optimization, purification, and characterization using novel Fusarium solani PVS1 for sustainability.Archives of microbiology · 2026Article
- Characterization and bioactivities of glutaminase-free L-asparaginase from Salinicola acorporae S4-41.Microbial cell factories · 2025Article
- Tapping the untapped: type II L-asparaginase from marine crustacean-derived Chryseomicrobium amylolyticum as a potential bio better for blood cancer therapy.Scientific reports · 2025Article
- Cytotoxicity of L-asparaginase from eucaryotic Cladosporium species against breast and colon cancer in vitro.Journal of the Egyptian National Cancer Institute · 2025Article
- Synergistic effects of ternary mixture formulation and process parameters optimization in a sequential approach for enhanced L-asparaginase production using agro-industrial wastes.Environmental science and pollution research international · 2024Article
- Molecular Modeling and Optimization of Type II E.coli l-Asparginase Activity by in silico Design and in vitro Site-directed Mutagenesis.The protein journal · 2023Article
- Characterization of L-asparaginase from Streptomyces koyangensis SK4 with acrylamide-minimizing potential in potato chips.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Response surface methodology based optimized production, purification, and characterization of L-asparaginase from Fusarium foetens.World journal of microbiology & biotechnology · 2023Article
- Improvement of recombinant L-Asparaginase production in3 Biotech · 2023Article
- Characterization and applications of glutaminase free L-asparaginase from indigenous Bacillus halotolerans ASN9.PloS one · 2023Article
- Characterization of a Novel L-Asparaginase fromFoods (Basel, Switzerland) · 2021Article
- Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado.Scientific reports · 2021Article
- Microbial L-asparaginase for Application in Acrylamide Mitigation from Food: Current Research Status and Future Perspectives.Microorganisms · 2021Review
- Production, purification, characterization, antioxidant and antiproliferative activities of extracellular L-asparaginase produced bySaudi journal of biological sciences · 2021Article
- What makes a good new therapeutic L-asparaginase?World journal of microbiology & biotechnology · 2019Review
- Penicillium and Talaromyces endophytes from Tillandsia catimbauensis, a bromeliad endemic in the Brazilian tropical dry forest, and their potential for L-asparaginase production.World journal of microbiology & biotechnology · 2018Article
- In silico analysis, molecular cloning, expression and characterization of l-asparaginase gene from3 Biotech · 2017Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-asparaginase from Cladosporium sp. grown on wheat bran by SSF was purified. Enzyme appeared to be a trimer with homodimer of 37 kDa and another 47 kDa amounting to total mass of 121 kDa as estimated by SDS-PAGE and 120 kDa on gel filtration column. The optimum temperature and pH of the enzyme were 30 °C and 6.3, respectively with Vmax of 4.44 μmol/mL/min and Km of 0.1 M. Substrate specificity studies indicated that, L-asparaginase has greater affinity towards L-asparagine with substrate hydrolysis efficiency (Vmax/Km ratio) eightfold higher than that of L-glutamine. L-asparaginase activity in presence of thiols studied showed decrease in Vmax and increase in Km, indicating nonessential mode of inactivation. Among the thiols tested, β-mercaptomethanol, exerted inhibitory effect, suggesting a critical role of disulphide linkages in maintaining a suitable conformation of the enzyme. Metal ions such as Ca(2+), Co(2+), Cu(2+), Mg(2+), Na(+), K(+) and Zn(2+) significantly affected enzyme activity whereas presence of Fe(3+), Pb(2+) and KI stimulated the activity. Detergents studied also enhanced L-asparaginase activity. In-vitro half-life of purified L-asparaginase in mammalian blood serum was 93.69 h. The enzyme inhibited acrylamide formation in potato chips by 96 % making it a potential candidate for food industry to reduce acrylamide content in starchy fried food commodities.
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