ArticleThe Journal of pathology2013
High-throughput RNA sequencing of a formalin-fixed, paraffin-embedded autopsy lung tissue sample from the 1918 influenza pandemic.
Article in The Journal of pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 74 citations in OpenAlex.
- Age-specific mortality patterns across influenza pandemics: evidence from all-cause mortality data across multiple populations.International journal of epidemiology · 2026Article
- Inhaled Angiopoietin-Like 4 Antisense Oligonucleotide Therapy for Lung Injury and Fibrosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Precursors to the 1918 pandemic: Finding Rickettsia felis and Mucor co-infection associated with a death in 1912.International journal of paleopathology · 2026Article
- Ancient DNA insights into diverse pathogens and their hosts.Nature reviews. Genetics · 2026Review
- Divergent macrophage responses to Influenza A virus andFrontiers in immunology · 2026Article
- Body Size and Risk of Death During the 1918 Influenza Pandemic in Alaska.American journal of human biology : the official journal of the Human Biology Council · 2025Article
- An ancient influenza genome from Switzerland allows deeper insights into host adaptation during the 1918 flu pandemic in Europe.BMC biology · 2025Article
- Reframing Formalin: A Molecular Opportunity Enabling Historical Epigenomics and Retrospective Gene Expression Studies.Molecular ecology resources · 2025Review
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- Historical RNA expression profiles from the extinct Tasmanian tiger.Genome research · 2023Article
- Historic and Prehistoric Epidemics: An Overview of Sources Available for the Study of Ancient Pathogens.Epidemiologia (Basel, Switzerland) · 2022Review
- Role of Neuropilin 1 in COVID-19 Patients with Acute Ischemic Stroke.Biomedicines · 2022Review
- 100-year-old pandemic flu viruses yield new genomes.Nature · 2022Article
- Review
- Archival influenza virus genomes from Europe reveal genomic variability during the 1918 pandemic.Nature communications · 2022Article
- Analysis of Non-Relapsed and Relapsed Adult Type Granulosa Cell Tumors Suggests Stable Transcriptomes during Tumor Progression.Current issues in molecular biology · 2022Article
- Comparable outcomes from long and short read random sequencing of total RNA for detection of pathogens in chicken respiratory samples.Frontiers in veterinary science · 2022Article
- Ubiquitous influenza A virus in Chilean swine before the H1N1pdm09 introduction.Transboundary and emerging diseases · 2021Article
- Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Genome Sequencing from Post-Mortem Formalin-Fixed, Paraffin-Embedded Lung Tissues.The Journal of molecular diagnostics : JMD · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Most biopsy and autopsy tissues are formalin-fixed and paraffin-embedded (FFPE), but this process leads to RNA degradation that limits gene expression analysis. The RNA genome of the 1918 pandemic influenza virus was previously determined in a 9-year effort by overlapping RT-PCR from post-mortem samples. Here, the full genome of the 1918 virus at 3000× coverage was determined in one high-throughput sequencing run of a library derived from total RNA of a 1918 FFPE sample after duplex-specific nuclease treatments. Bacterial sequences associated with secondary bacterial pneumonias were also detected. Host transcripts were well represented in the library. Compared to a 2009 pandemic influenza virus FFPE post-mortem library, the 1918 sample showed significant enrichment for host defence and cell death response genes, concordant with prior animal studies. This methodological approach should assist in the analysis of FFPE tissue samples isolated over the past century from a variety of diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.