ArticleMethods in molecular biology (Clifton, N.J.)2013
Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 181 citations in OpenAlex.
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- Embedding muscle fibers in hydrogel improves viability and preserves contractile function during prolonged ex vivo culture.The Journal of general physiology · 2026Article
- Isolation and Expansion of Muscle Satellite Cells Using the Ice-Cold Treatment Method.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Combining In Vivo 2-Photon Imaging with Photoactivatable Fluorescent Labeling Shows Low Rates of Mitochondrial Dynamics in Skeletal Muscle.Medicine and science in sports and exercise · 2025Article
- Embedding muscle fibers in hydrogel improves viability and preserves contractile function during prolongedbioRxiv : the preprint server for biology · 2025Article
- Ankyrin-B modulates mitochondrial fission in skeletal muscle and is required for optimal endurance exercise capacity.Nature communications · 2025Article
- SMN depletion impairs skeletal muscle formation and maturation in a mouse model of SMA.Human molecular genetics · 2025Article
- Derivation and long-term maintenance of porcine skeletal muscle progenitor cells.Scientific reports · 2024Article
- Voluntary wheel running improves molecular and functional deficits in a murine model of facioscapulohumeral muscular dystrophy.iScience · 2024Article
- Article
- Advanced Methodology for Rapid Isolation of Single Myofibers from Flexor Digitorum Brevis Muscle.Tissue engineering. Part C, Methods · 2023Article
- Gαi2 regulates the adult myogenesis of masticatory muscle satellite cells.Journal of cellular and molecular medicine · 2023Article
- Intercellular Adhesion Molecule-1 Enhances Myonuclear Transcription during Injury-Induced Muscle Regeneration.International journal of molecular sciences · 2022Article
- ATAC-Seq of a Single Myofiber fromBio-protocol · 2022Article
- Laminin-221-derived recombinant fragment facilitates isolation of cultured skeletal myoblasts.Regenerative therapy · 2022Article
- The effect of hypoxia on myogenic differentiation and multipotency of the skeletal muscle-derived stem cells in mice.Stem cell research & therapy · 2022Article
- Positional Context of Myonuclear Transcription During Injury-Induced Muscle Regeneration.Frontiers in physiology · 2022Article
- Quantification of Muscle Stem Cell Differentiation Using Live-Cell Imaging and Eccentricity Measures.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Lipocalin 2 increases after high-intensity exercise in humans and influences muscle gene expression and differentiation in mice.Journal of cellular physiology · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Multinucleated myofibers are the functional contractile units of skeletal muscle. In adult muscle, mononuclear satellite cells, located between the basal lamina and the plasmalemma of the myofiber, are the primary myogenic stem cells. This chapter describes protocols for isolation, culturing, and immunostaining of myofibers from mouse skeletal muscle. Myofibers are isolated intact and retain their associated satellite cells. The first protocol discusses myofiber isolation from the flexor digitorum brevis (FDB) muscle. These short myofibers are cultured in dishes coated with PureCol collagen (formerly known as Vitrogen) using a serum replacement medium. Employing such culture conditions, satellite cells remain associated with the myofibers, undergoing proliferation and differentiation on the myofiber surface. The second protocol discusses the isolation of longer myofibers from the extensor digitorum longus (EDL) muscle. Different from the FDB preparation, where multiple myofibers are processed together, the longer EDL myofibers are typically processed and cultured individually in dishes coated with Matrigel using a growth factor rich medium. Under these conditions, satellite cells initially remain associated with the parent myofiber and later migrate away, giving rise to proliferating and differentiating progeny. Myofibers from other types of muscles, such as diaphragm, masseter, and extraocular muscles can also be isolated and analyzed using protocols described herein. Overall, cultures of isolated myofibers provide essential tools for studying the interplay between the parent myofiber and its associated satellite cells. The current chapter provides background, procedural, and reagent updates, and step-by-step images of FDB and EDL muscle isolations, not included in our 2005 publication in this series.
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