ArticlePharmaceutical research2013
Involvement of a novel organic cation transporter in verapamil transport across the inner blood-retinal barrier.
Article in Pharmaceutical research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 65 citations in OpenAlex.
- Advances in Modeling the Inner Blood-Retinal Barrier: From Static Tissue Cell Cultures to Microphysiological Systems.Pharmaceuticals (Basel, Switzerland) · 2025Review
- The putative proton-coupled organic cation antiporter is involved in uptake of triptans into human brain capillary endothelial cells.Fluids and barriers of the CNS · 2024Article
- Proteomics-Based Transporter Identification by the PICK Method: Involvement of TM7SF3 and LHFPL6 in Proton-Coupled Organic Cation Antiport at the Blood-Brain Barrier.Pharmaceutics · 2022Article
- Total Synthesis of Decahydroquinoline Poison Frog Alkaloids ent-Molecules (Basel, Switzerland) · 2021Article
- Comprehensive Evidence of Carrier-Mediated Distribution of Amantadine to the Retina across the Blood-Retinal Barrier in Rats.Pharmaceutics · 2021Article
- ABCB1 and ABCG2 Together Limit the Distribution of ABCB1/ABCG2 Substrates to the Human Retina and theFrontiers in pharmacology · 2021Article
- Article
- Involvement of a Novel Organic Cation Transporter in Paeonol Transport Across the Blood-Brain Barrier.Biomolecules & therapeutics · 2019Article
- Roles of Drug Transporters in Blood-Retinal Barrier.Advances in experimental medicine and biology · 2019Review
- P-glycoprotein Restricts Ocular Penetration of Loperamide across the Blood-Ocular Barriers: a Comparative Study in Mdr1a Knock-out and Wild Type Sprague Dawley Rats.AAPS PharmSciTech · 2018Article
- Blood-to-Retina Transport of Fluorescence-Labeled Verapamil at the Blood-Retinal Barrier.Pharmaceutical research · 2018Article
- Imperatorin is Transported through Blood-Brain Barrier by Carrier-Mediated Transporters.Biomolecules & therapeutics · 2017Article
- Blood-brain and retinal barriers show dissimilar ABC transporter impacts and concealed effect of P-glycoprotein on a novel verapamil influx carrier.British journal of pharmacology · 2016Article
- Fitting Transporter Activities to Cellular Drug Concentrations and Fluxes: Why the Bumblebee Can Fly.Trends in pharmacological sciences · 2015Article
- pH Dependent but not P-gp Dependent Bidirectional Transport Study of S-propranolol: The Importance of Passive Diffusion.Pharmaceutical research · 2015Article
- The putative P-gp inhibitor telmisartan does not affect the transcellular permeability and cellular uptake of the calcium channel antagonist verapamil in the P-glycoprotein expressing cell line MDCK II MDR1.Pharmacology research & perspectives · 2015Article
- How drugs get into cells: tested and testable predictions to help discriminate between transporter-mediated uptake and lipoidal bilayer diffusion.Frontiers in pharmacology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo clarify the transport and inhibition characteristics involved in verapamil transport across the inner blood-retinal barrier (inner BRB).
methodsThe transport of [(3)H]verapamil across the inner BRB was investigated using retinal uptake index and integration plot analyses in rats. The detailed transport characteristics were studied using TR-iBRB2 cells, a conditionally immortalized rat retinal capillary endothelial cell line that is an in vitro model of the inner BRB.
resultsThe apparent influx permeability clearance of [(3)H]verapamil was 614 μL/(min·g retina), which is 4.7-fold greater than that of brain. The retinal uptake of [(3)H]verapamil was slightly increased by 3 mM verapamil and 10 mM qunidine and inhibited by 40 mM pyrilamine, supporting the carrier-mediated efflux and influx transport of verapamil across the inner BRB. TR-iBRB2 cells exhibited a concentration-dependent uptake of [(3)H]verapamil with a K (m) of 61.9 μM, and the uptake was inhibited by several cations, such as pyrilamine, exhibiting a different profile from the identified transporters. These transport properties suggest that verapamil transport at the inner BRB takes place via a novel organic cation transporter.
conclusionsOur findings suggest that a novel organic cation transporter is involved in verapamil transport from the blood to the retina across the inner BRB.
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