ArticlePlant cell reports2013
Variations in genomic DNA methylation during the long-term in vitro proliferation of oil palm embryogenic suspension cultures.
Article in Plant cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Exploring the Significance of Somaclonal Variations in Horticultural Crops.Molecular biotechnology · 2025Review
- Small molecules, enormous functions: potential approach for overcoming bottlenecks in embryogenic tissue induction and maintenance in conifers.Horticulture research · 2024Review
- Comparative restriction enzyme analysis of methylation (CREAM) reveals methylome variability within a clonalFrontiers in plant science · 2024Article
- Article
- DNA cytosine methylation dynamics and functional roles in horticultural crops.Horticulture research · 2023Article
- Effect of Explant Source on Phenotypic Changes of In Vitro Grown Cannabis Plantlets over Multiple Subcultures.Biology · 2023Article
- Genetic and epigenetic modes of the regulation of somatic embryogenesis: a review.Biologia futura · 2022Review
- Oil Palm Breeding in the Modern Era: Challenges and Opportunities.Plants (Basel, Switzerland) · 2022Review
- DNA Methylome and LncRNAome Analysis Provide Insights Into Mechanisms of Genome-Dosage Effects in Autotetraploid Cassava.Frontiers in plant science · 2022Article
- Genome-wide high-resolution mapping of DNA methylation reveals epigenetic variation in the offspring of sexual and asexual propagation in Robinia pseudoacacia.Plant cell reports · 2021Article
- Elevated transcription of transposable elements is accompanied by het-siRNA-driven de novo DNA methylation in grapevine embryogenic callus.BMC genomics · 2021Article
- Comparative Study of the Genetic and Biochemical Variability ofInternational journal of molecular sciences · 2021Article
- Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture.Journal of biological research (Thessalonike, Greece) · 2021Article
- Hypermethylation of Auxin-Responsive Motifs in the Promoters of the Transcription Factor Genes Accompanies the Somatic Embryogenesis Induction in Arabidopsis.International journal of molecular sciences · 2020Article
- Development of a fast and user-friendly cryopreservation protocol for sweet potato genetic resources.Scientific reports · 2020Article
- Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants.Plants (Basel, Switzerland) · 2020Article
- Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration.Plants (Basel, Switzerland) · 2020Review
- Tissue Culture of Oil Palm: Finding the Balance Between Mass Propagation and Somaclonal Variation.Frontiers in plant science · 2019Review
- 5-Azacytidine: A Promoter of Epigenetic Changes in the Quest to Improve Plant Somatic Embryogenesis.International journal of molecular sciences · 2018Review
- siRNAs regulate DNA methylation and interfere with gene and lncRNA expression in the heterozygous polyploid switchgrass.Biotechnology for biofuels · 2018Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThe long-term proliferation of embryogenic cell suspensions of oil palm is associated with changes in both genomic methylation rates and embryogenic capacities. In the aim of exploring the relationship between epigenetic stability and the long-term in vitro proliferation of plant tissues, we have studied changes in genomic DNA methylation levels in embryogenic suspensions of oil palm (Elaeis guineensis Jacq.). Five embryogenic callus lines were obtained from selected hybrid seeds and then proliferated as suspension cultures. Each clonal line obtained from a single genotype was subdivided into three independent subclonal lines. Once established, cultures proliferated for 12 months and genomic DNA was sampled at 4 months intervals for the estimation of global DNA methylation rates through high performance liquid chromatography (HPLC) quantitation of deoxynucleosides. Our results show that in vitro proliferation induces DNA hypermethylation in a time-dependent fashion. Moreover, this trend is statistically significant in several clonal lines and shared between subclonal lines originating from the same genotype. Interestingly, the only clonal line undergoing loss of genomic methylation in the course of proliferation has been found unable to generate somatic embryos. We discuss the possible implications of genome-wide DNA methylation changes in proliferating cells with a view to the maintenance of genomic and epigenomic stability.
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