Evidence map›Paper›PMID 23179209›Full record

ArticleDer Pathologe2013

[Round robin test for detection of genomic aberrations in non-Hodgkin lymphoma by in situ hybridization].

T F E Barth, L Floßbach, H-W Bernd, R Bob, M Buck, S B Cogliatti, A C Feller, M L Hansmann, S Hartmann, H Horn and 8 more

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Der Pathologe, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 8 institutions in 2 countries.

T F E BarthInstitut für Pathologie, Universitätsklinikum Ulm, Albert-Einstein-Allee 23, Ulm, Germany. thomas.barth@uniklinik-ulm.de
L Floßbach
H-W Bernd
R Bob
M Buck
S B Cogliatti
A C Feller
M L Hansmann
S Hartmann
H Horn
W Klapper
D Kradolfer
T Mattfeldt
P Möller
A Rosenwald
H Stein
C Thorns
G Ott
University Hospital Ulm · DEUniversity of Lübeck · DEGoethe University Frankfurt · DEKantonsspital St. Gallen · CHPathodiagnostik Berlin · DERobert Bosch Hospital · DEUniversitäts Hautklinik Kiel · DEUniversity of Würzburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe detection of characteristic genomic aberrations by fluorescence in situ hybridization (FISH) has a high diagnostic impact on lymphomas according to the World Health Organization (WHO). To investigate the reproducibility of non-isotopic ISH results a multicenter trial was carried out involving eight institutes for hematopathology. MATERIAL AND

methodsAnalyses were performed on two diffuse large B-cell lymphomas (DLBCL) without known aberrations, on one follicular lymphoma with a IGH/BCL2 translocation and BCL6 split and on two B-cell lymphomas intermediate between DLBCL and Burkitt's lymphoma with c-MYC and BCL2 rearrangements, one with an additional BCL6 split. Break-apart probes for BCL6 and c-MYC, as well as fusion probes for the c-MYC/IGH and the IGH/BCL2 translocations were used.

resultsAll aberrations were correctly detected by all centres and no false positive or false negative results were obtained. The numbers of positive cells varied from 25% to 94%. Pearson's correlation coefficient between the centres was always > 0.8.

conclusionsThe ISH analysis of recurrent genomic aberrations in formalin-fixed paraffin-embedded (FFPE) tissue is a highly reproducible technique which yields substantial additive help for lymphoma diagnostics.

Indexed as

Chromosome AberrationsBiomarkers, TumorBurkitt LymphomaDiagnosis, DifferentialDNA-Binding ProteinsGenes, mycHumansImmunoglobulin Heavy ChainsIn Situ HybridizationIn Situ Hybridization, FluorescenceLymphoma, FollicularLymphoma, Large B-Cell, DiffuseLymphoma, Non-HodgkinProto-Oncogene Proteins c-bcl-6Quality Assurance, Health CareReproducibility of ResultsBCL6 protein, humanBiomarkers, TumorDNA-Binding ProteinsImmunoglobulin Heavy ChainsProto-Oncogene Proteins c-bcl-6

Identifiers

PMID23179209
OpenAlexW966467912

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.