ArticleInternational journal for parasitology2013
Proteomic analysis of the Theileria annulata schizont.
Article in International journal for parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Stage-specific proteomic and phosphoproteomic remodeling in Theileria annulata.Parasitology research · 2026Article
- Investigation of carbohydrate-based molecules ofFrontiers in veterinary science · 2026Article
- Systematic proteomic and small RNA profiling of extracellular vesicles from cattle infected with a naturally occurring buparvaquone-resistant strain of Theileria annulata and from uninfected controls.Parasites & vectors · 2025Article
- Comparative proteomics and structure-based approach to unravel the therapeutic drug target of Theileria species.Journal, genetic engineering & biotechnology · 2025Article
- Identification of a Novel Interaction betweenMicrobiology spectrum · 2023Article
- Article
- Characterisation of thePathogens (Basel, Switzerland) · 2022Article
- Theileria annulata SVSP455 interacts with host HSP60.Parasites & vectors · 2022Article
- Discovery and evaluation of inhibitory activity and mechanism of arylcoumarin derivatives on Theileria annulata enolase by in vitro and molecular docking studies.Molecular diversity · 2020Article
- Re-annotation of the Theileria parva genome refines 53% of the proteome and uncovers essential components of N-glycosylation, a conserved pathway in many organisms.BMC genomics · 2020Article
- Comparative Transcriptomics of the Bovine Apicomplexan ParasiteFrontiers in veterinary science · 2020Article
- Characterization of the Theileria parva sporozoite proteome.International journal for parasitology · 2018Article
- Isolation and purification of glycosylphosphatidylinositols (GPIs) in the schizont stage of Theileria annulata and determination of antibody response to GPI anchors in vaccinated and infected animals.Parasites & vectors · 2018Article
- Immuno-informatics Analysis to Identify Novel Vaccine Candidates and Design of a Multi-Epitope Based Vaccine Candidate AgainstFrontiers in immunology · 2018Article
- Biochemical and in silico Characterization of Recombinant L-Lactate Dehydrogenase of Theileria annulata.Molecular biotechnology · 2016Article
- Theileria parasites secrete a prolyl isomerase to maintain host leukocyte transformation.Nature · 2015Article
- A quantitative reverse-transcriptase PCR assay for the assessment of drug activities against intracellular Theileria annulata schizonts.International journal for parasitology. Drugs and drug resistance · 2014Article
- Cloning of intron-removed enolase gene and expression, purification, kinetic characterization of the enzyme from Theileria annulata.Molecular biotechnology · 2014Article
- Cell cycle-dependent phosphorylation of Theileria annulata schizont surface proteins.PloS one · 2014Article
- Recruitment of EB1, a master regulator of microtubule dynamics, to the surface of the Theileria annulata schizont.PLoS pathogens · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The apicomplexan parasite, Theileria annulata, is the causative agent of tropical theileriosis, a devastating lymphoproliferative disease of cattle. The schizont stage transforms bovine leukocytes and provides an intriguing model to study host/pathogen interactions. The genome of T. annulata has been sequenced and transcriptomic data are rapidly accumulating. In contrast, little is known about the proteome of the schizont, the pathogenic, transforming life cycle stage of the parasite. Using one-dimensional (1-D) gel LC-MS/MS, a proteomic analysis of purified T. annulata schizonts was carried out. In whole parasite lysates, 645 proteins were identified. Proteins with transmembrane domains (TMDs) were under-represented and no proteins with more than four TMDs could be detected. To tackle this problem, Triton X-114 treatment was applied, which facilitates the extraction of membrane proteins, followed by 1-D gel LC-MS/MS. This resulted in the identification of an additional 153 proteins. Half of those had one or more TMD and 30 proteins with more than four TMDs were identified. This demonstrates that Triton X-114 treatment can provide a valuable additional tool for the identification of new membrane proteins in proteomic studies. With two exceptions, all proteins involved in glycolysis and the citric acid cycle were identified. For at least 29% of identified proteins, the corresponding transcripts were not present in the existing expressed sequence tag databases. The proteomics data were integrated into the publicly accessible database resource at EuPathDB (www.eupathdb.org) so that mass spectrometry-based protein expression evidence for T. annulata can be queried alongside transcriptional and other genomics data available for these parasites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.