ArticleThe Journal of molecular diagnostics : JMD2013
Impact of mutation type and amplicon characteristics on genetic diversity measures generated using a high-resolution melting diversity assay.
Article in The Journal of molecular diagnostics : JMD, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 20 citations in OpenAlex.
- Association of HIV diversity and virologic outcomes in early antiretroviral treatment: HPTN 052.PloS one · 2017Trial
- Haplotype and Presence-Absence Variation Analysis Using High-Resolution Melting (HRM) Analysis for Genotyping Genes of Interest.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Comparison of genotyping assays for detection of targeted CRISPR/Cas mutagenesis in highly polyploid sugarcane.Frontiers in genome editing · 2024Article
- Evaluation of Phylogenetic Methods for Inferring the Direction of Human Immunodeficiency Virus (HIV) Transmission: HIV Prevention Trials Network (HPTN) 052.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Detection of 'Candidatus Phytoplasma solani' in roots from Bois noir symptomatic and recovered grapevines.Scientific reports · 2019Article
- High Resolution Melting (HRM) for High-Throughput Genotyping-Limitations and Caveats in Practical Case Studies.International journal of molecular sciences · 2017Article
- Nested Machine Learning Facilitates Increased Sequence Content for Large-Scale Automated High Resolution Melt Genotyping.Scientific reports · 2016Article
- Analysis of HIV Diversity in HIV-Infected Black Men Who Have Sex with Men (HPTN 061).PloS one · 2016Article
- Review
- High-resolution melting (HRM) re-analysis of a polyposis patients cohort reveals previously undetected heterozygous and mosaic APC gene mutations.Familial cancer · 2015Article
- Article
- HIV diversity as a biomarker for HIV incidence estimation: including a high-resolution melting diversity assay in a multiassay algorithm.Journal of clinical microbiology · 2014Article
- A comparison of two measures of HIV diversity in multi-assay algorithms for HIV incidence estimation.PloS one · 2014Article
- Article
- Use of a high resolution melting assay to analyze HIV diversity in HIV-infected Ugandan children.The Pediatric infectious disease journal · 2012Article
- Article
- High-resolution melting-based TILLING of γ ray-induced mutations in rice.Journal of Zhejiang University. Science. BArticle
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
We adapted high-resolution melting (HRM) technology to measure genetic diversity without sequencing. Diversity is measured as a single numeric HRM score. Herein, we determined the impact of mutation types and amplicon characteristics on HRM diversity scores. Plasmids were generated with single-base changes, insertions, and deletions. Different primer sets were used to vary the position of mutations within amplicons. Plasmids and plasmid mixtures were analyzed to determine the impact of mutation type, position, and concentration on HRM scores. The impact of amplicon length and G/C content on HRM scores was also evaluated. Different mutation types affected HRM scores to varying degrees (1-bp deletion < 1-bp change < 3-bp insertion < 9-bp insertion). The impact of mutations on HRM scores was influenced by amplicon length and the position of the mutation within the amplicon. Mutations were detected at concentrations of 5% to 95%, with the greatest impact at 50%. The G/C content altered melting temperature values of amplicons but had no impact on HRM scores. These data are relevant to the design of assays that measure genetic diversity using HRM technology.
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