ArticleBMC microbiology2012
Highly specific fiber optic immunosensor coupled with immunomagnetic separation for detection of low levels of Listeria monocytogenes and L. ivanovii.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
- Biosensing Strategies to Monitor Contaminants and Additives on Fish, Meat, Poultry, and Related Products.Biosensors · 2025Review
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- Optical Immunosensor for the Detection ofACS omega · 2023Article
- Identification ofPathogens (Basel, Switzerland) · 2023Article
- Molecular Targets for Foodborne Pathogenic Bacteria Detection.Pathogens (Basel, Switzerland) · 2023Review
- Combined antibodies against internalins A and B proteins have potential application in immunoassay for detection ofJournal of food science and technology · 2023Article
- Petri-plate, bacteria, and laser optical scattering sensor.Frontiers in cellular and infection microbiology · 2022Review
- Phage Display-Derived Monoclonal Antibodies Against Internalins A and B Allow Specific Detection ofFrontiers in public health · 2022Article
- Antibody- and nucleic acid-based lateral flow immunoassay for Listeria monocytogenes detection.Analytical and bioanalytical chemistry · 2021Review
- Biofilm-isolated Listeria monocytogenes exhibits reduced systemic dissemination at the early (12-24 h) stage of infection in a mouse model.NPJ biofilms and microbiomes · 2021Article
- Advances in Optical Detection of Human-Associated Pathogenic Bacteria.Molecules (Basel, Switzerland) · 2020Review
- Pyruvate dehydrogenase complex-enzyme 2, a new target for Listeria spp. detection identified using combined phage display technologies.Scientific reports · 2020Article
- Lactobacillus casei expressing Internalins A and B reduces Listeria monocytogenes interaction with Caco-2 cells in vitro.Microbial biotechnology · 2019Article
- Characterization and Discrimination of Gram-Positive Bacteria Using Raman Spectroscopy with the Aid of Principal Component Analysis.Nanomaterials (Basel, Switzerland) · 2017Article
- Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species.PloS one · 2016Article
- Quantification of Gram-positive bacteria: adaptation and evaluation of a preparation strategy using high amounts of clinical tissue.BMC veterinary research · 2014Article
- Article
- Rapid and sensitive detection of Listeria ivanovii by loop-mediated isothermal amplification of the smcL gene.PloS one · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundImmunomagnetic separation (IMS) and immunoassays are widely used for pathogen detection. However, novel technology platforms with highly selective antibodies are essential to improve detection sensitivity, specificity and performance. In this study, monoclonal antibodies (MAbs) against Internalin A (InlA) and p30 were generated and used on paramagnetic beads of varying diameters for concentration, as well as on fiber-optic sensor for detection.
resultsAnti-InlA MAb-2D12 (IgG2a subclass) was specific for Listeria monocytogenes and L. ivanovii, and p30-specific MAb-3F8 (IgM) was specific for the genus Listeria. At all bacterial concentrations (10³-10⁸ CFU/mL) tested in the IMS assay; the 1-μm diameter MyOne beads had significantly higher capture efficiency (P < 0.05) than the 2.8-μm diameter M-280 beads with both antibodies. The highest capture efficiency for MyOne-2D12 (49.2% for 10⁵ CFU/mL) was significantly higher (P < 0.05) than that of MyOne-3F8 (16.6 %) and Dynabeads anti-Listeria antibody (9 %). Furthermore, capture efficiency for MyOne-2D12 was highly specific for L. monocytogenes and L. ivanovii. Subsequently, we captured L. monocytogenes by MyOne-2D12 and MyOne-3F8 from hotdogs inoculated with mono- or co-cultures of L. monocytogenes and L. innocua (10-40 CFU/g), enriched for 18 h and detected by fiber-optic sensor and confirmed by plating, light-scattering, and qPCR assays. The detection limit for L. monocytogenes and L. ivanovii by the fiber-optic immunosensor was 3 × 10² CFU/mL using MAb-2D12 as capture and reporter antibody. Selective media plating, light-scattering, and qPCR assays confirmed the IMS and fiber-optic results.
conclusionsIMS coupled with a fiber-optic sensor using anti-InlA MAb is highly specific for L. monocytogenes and L. ivanovii and enabled detection of these pathogens at low levels from buffer or food.
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