ArticleBMC microbiology2012
Investigations into genome diversity of Haemophilus influenzae using whole genome sequencing of clinical isolates and laboratory transformants.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species.Scientific reports · 2018Trial
- Development and implementation of a core genome multilocus sequence typing scheme forMicrobial genomics · 2024Article
- Article
- Multiple interspecies recombination events documented by whole-genome sequencing in multidrug-resistantAccess microbiology · 2024Article
- Identifying Bacterial Airways Infection in Stable Severe Asthma Using Oxford Nanopore Sequencing Technologies.Microbiology spectrum · 2022Article
- Learning from -omics strategies applied to uncoverComputational and structural biotechnology journal · 2021Review
- Whole-genome analyses reveal gene content differences between nontypeableMicrobial genomics · 2020Article
- Article
- Molecular Signatures of Non-typeableFrontiers in microbiology · 2019Article
- The return of Pfeiffer's bacillus: Rising incidence of ampicillin resistance in Haemophilus influenzae.Microbial genomics · 2018Review
- Article
- Virulence factor activity relationships (VFARs): a bioinformatics perspective.Environmental science. Processes & impacts · 2017Review
- Targeted single molecule mutation detection with massively parallel sequencing.Nucleic acids research · 2016Article
- 3D Reconstruction of the Human Airway Mucosa In Vitro as an Experimental Model to Study NTHi Infections.PloS one · 2016Article
- Expression of IgA Proteases by Haemophilus influenzae in the Respiratory Tract of Adults With Chronic Obstructive Pulmonary Disease.The Journal of infectious diseases · 2015Article
- Vaccines for Nontypeable Haemophilus influenzae: the Future Is Now.Clinical and vaccine immunology : CVI · 2015Review
- Comparative whole-genome analysis of clinical isolates reveals characteristic architecture of Mycobacterium tuberculosis pangenome.PloS one · 2015Article
- Multilocus sequence typing and ftsI sequencing: a powerful tool for surveillance of penicillin-binding protein 3-mediated beta-lactam resistance in nontypeable Haemophilus influenzae.BMC microbiology · 2014Article
- Extensive cotransformation of natural variation into chromosomes of naturally competent Haemophilus influenzae.G3 (Bethesda, Md.) · 2014Article
- Genome sequencing of disease and carriage isolates of nontypeable Haemophilus influenzae identifies discrete population structure.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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Authors and funding
5 authors.
Funding
Abstract
backgroundHaemophilus influenzae is an important human commensal pathogen associated with significant levels of disease. High-throughput DNA sequencing was used to investigate differences in genome content within this species.
resultsGenomic DNA sequence was obtained from 85 strains of H. influenzae and from other related species, selected based on geographical site of isolation, disease association and documented genotypic and phenotypic differences. When compared by Mauve alignment these indicated groupings of H. influenzae that were consistent with previously published analyses; capsule expressing strains fell into two distinct groups and those of serotype b (Hib) were found in two closely positioned lineages. For 18 Hib strains representing both lineages we found many discrete regions (up to 40% of the total genome) displaying sequence variation when compared to a common reference strain. Evidence that this naturally occurring pattern of inter-strain variation in H. influenzae can be mediated by transformation was obtained through sequencing DNA obtained from a pool of 200 independent transformants of a recipient (strain Rd) using donor DNA from a heterologous Hib strain (Eagan).
conclusionMuch of the inter-strain variation in genome sequence in H. influenzae is likely the result of inter-strain exchanges of DNA, most plausibly through transformation.
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