ArticleThe Plant cell2012
Protein-protein and protein-membrane associations in the lignin pathway.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 145 citations in OpenAlex.
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- Enhancing cross-organelle coordination to advance plant cytochrome P450 functionality in yeast.Science advances · 2025Article
- Elucidating the enzyme network driving Amaryllidaceae alkaloids biosynthesis in Leucojum aestivum.Plant biotechnology journal · 2025Article
- Identification of GiOMT gene family in Glycyrrhiza inflata bat and expression analysis under UV-B stresses.BMC genomics · 2024Article
- Evolutionary analysis of anthocyanin biosynthetic genes: insights into abiotic stress adaptation.Plant molecular biology · 2024Review
- Phenolic amides (avenanthramides) in oats - an update review.Bioengineered · 2024Review
- An ancient role for CYP73 monooxygenases in phenylpropanoid biosynthesis and embryophyte development.The EMBO journal · 2024Article
- A century of studying plant secondary metabolism-From "what?" to "where, how, and why?"Plant physiology · 2024Review
- Deciphering the intricate hierarchical gene regulatory network: unraveling multi-level regulation and modifications driving secondary cell wall formation.Horticulture research · 2024Article
- Immunoprecipitation and FRET-FLIM to Determine Metabolons on the Plant ER.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Global organization of phenylpropanoid and anthocyanin pathways revealed by proximity labeling of trans-cinnamic acid 4-hydroxylase inFrontiers in plant science · 2024Article
- Raspberry Ketone Accumulation inJournal of agricultural and food chemistry · 2023Article
- Altered profile of floral volatiles and lignin content by down-regulation of Caffeoyl Shikimate Esterase in Petunia.BMC plant biology · 2023Article
- Pathogen-induced autophagy regulates monolignol transport and lignin formation in plant immunity.Autophagy · 2023Article
- Is a spice missing from the recipe? The intra-cellular localization of vanillin biosynthesis needs further investigations.Plant biology (Stuttgart, Germany) · 2023Article
- Cloning and Characterization of Two NovelInternational journal of molecular sciences · 2022Article
- Genome-edited rice deficient in two 4-COUMARATE:COENZYME A LIGASE genes displays diverse lignin alterations.Plant physiology · 2022Article
- UGT72, a Major Glycosyltransferase Family for Flavonoid and Monolignol Homeostasis in Plants.Biology · 2022Review
- Interactome of Arabidopsis Thaliana.Plants (Basel, Switzerland) · 2022Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Supramolecular organization of enzymes is proposed to orchestrate metabolic complexity and help channel intermediates in different pathways. Phenylpropanoid metabolism has to direct up to 30% of the carbon fixed by plants to the biosynthesis of lignin precursors. Effective coupling of the enzymes in the pathway thus seems to be required. Subcellular localization, mobility, protein-protein, and protein-membrane interactions of four consecutive enzymes around the main branch point leading to lignin precursors was investigated in leaf tissues of Nicotiana benthamiana and cells of Arabidopsis thaliana. CYP73A5 and CYP98A3, the two Arabidopsis cytochrome P450s (P450s) catalyzing para- and meta-hydroxylations of the phenolic ring of monolignols were found to colocalize in the endoplasmic reticulum (ER) and to form homo- and heteromers. They moved along with the fast remodeling plant ER, but their lateral diffusion on the ER surface was restricted, likely due to association with other ER proteins. The connecting soluble enzyme hydroxycinnamoyltransferase (HCT), was found partially associated with the ER. Both HCT and the 4-coumaroyl-CoA ligase relocalized closer to the membrane upon P450 expression. Fluorescence lifetime imaging microscopy supports P450 colocalization and interaction with the soluble proteins, enhanced by the expression of the partner proteins. Protein relocalization was further enhanced in tissues undergoing wound repair. CYP98A3 was the most effective in driving protein association.
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