ArticleNucleic acids research2013
Las1 interacts with Grc3 polynucleotide kinase and is required for ribosome synthesis in Saccharomyces cerevisiae.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 50 citations in OpenAlex.
- Many paths to destruction: Family-specific turnover and stress responses for tRNA introns.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A trypanosome-specific complex mediates late-stage processing of cytosolic LSU rRNA.Nucleic acids research · 2025Article
- MANY PATHS TO DESTRUCTION: FAMILY-SPECIFIC TURNOVER AND STRESS RESPONSES FOR TRNA INTRONS.bioRxiv : the preprint server for biology · 2025Article
- Independent neofunctionalization of Dxo1 inRNA (New York, N.Y.) · 2024Article
- USP36 SUMOylates Las1L and Promotes Its Function in Pre-Ribosomal RNA ITS2 Processing.Cancer research communications · 2024Article
- High resolution landscape of ribosomal RNA processing and surveillance.Nucleic acids research · 2024Article
- The novel pre-rRNA detection workflow "Riboprobing" allows simple identification of undescribed RNA species.RNA (New York, N.Y.) · 2024Article
- Structural and mechanistic insights into ribosomal ITS2 RNA processing by nuclease-kinase machinery.eLife · 2024Article
- Structural insights into coordinating 5S RNP rotation with ITS2 pre-RNA processing during ribosome formation.EMBO reports · 2023Article
- Systematic Analysis of Diverse Polynucleotide Kinase Clp1 Family Proteins in Eukaryotes: Three Unique Clp1 Proteins of Trypanosoma brucei.Journal of molecular evolution · 2023Article
- Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S biogenesis.EMBO reports · 2023Article
- Ribosome biogenesis factors-from names to functions.The EMBO journal · 2023Review
- Cryo-EM reveals the architecture of the PELP1-WDR18 molecular scaffold.Nature communications · 2022Article
- p53 induces a survival transcriptional response after nucleolar stress.Molecular biology of the cell · 2021Article
- Structural overview of macromolecular machines involved in ribosome biogenesis.Current opinion in structural biology · 2021Review
- HEPN RNases - an emerging class of functionally distinct RNA processing and degradation enzymes.Critical reviews in biochemistry and molecular biology · 2021Review
- Mutational Analysis of the Nsa2 N-Terminus Reveals Its Essential Role in Ribosomal 60S Subunit Assembly.International journal of molecular sciences · 2020Article
- A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin.eLife · 2020Article
- Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates.Journal of visualized experiments : JoVE · 2020Article
- It takes two (Las1 HEPN endoribonuclease domains) to cut RNA correctly.The Journal of biological chemistry · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Ribosome biogenesis is a multi-step process that couples cell growth with cell proliferation. Although several large-scale analysis of pre-ribosomal particles have identified numerous trans-acting factors involved in this process, many proteins involved in pre-rRNA processing and ribosomal subunit maturation have yet to be identified. Las1 was originally identified in Saccharomyces cerevisiae as a protein involved in cell morphogenesis. We previously demonstrated that the human homolog, Las1L, is required for efficient ITS2 rRNA processing and synthesis of the 60S ribosomal subunit. Here, we report that the functions of Las1 in ribosome biogenesis are also conserved in S. cerevisiae. Depletion of Las1 led to the accumulation of both the 27S and 7S rRNA intermediates and impaired the synthesis of the 60S subunit. We show that Las1 co-precipitates mainly with the 27S rRNA and associates with an Nsa1 and Rix1-containing pre-60S particle. We further identify Grc3 as a major Las1-interacting protein. We demonstrate that the kinase activity of Grc3 is required for efficient pre-rRNA processing and that depletion of Grc3 leads to rRNA processing defects similar to the ones observed in Las1-depleted cells. We propose that Las1 and Grc3 function together in a conserved mechanism to modulate rRNA processing and eukaryotic ribosome biogenesis.
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