ArticleMicrobiology (Reading, England)2013
The nucleoid-associated protein HUβ affects global gene expression in Porphyromonas gingivalis.
Article in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 28 citations in OpenAlex.
- Nucleoid-associated protein HU constrains transcription elongation to influence cellular functions, virulence, and drug tolerance in Mycobacterium tuberculosis.Nucleic acids research · 2026Article
- Transcription regulator TrtA modulates oxygen stress response inApplied and environmental microbiology · 2025Article
- Phenotypic Characterization of Null Mutants of ΔcbpA and ΔcbpB in S. enterica Serovar Typhimurium.Current microbiology · 2025Article
- The nucleoid protein HU positively regulates the expression of type VI secretion systems inmSphere · 2024Article
- Bacteriophage SPO1 protein Gp46 suppresses functions of HU protein inFrontiers in microbiology · 2023Article
- Growth ofJournal of oral microbiology · 2023Article
- Arginine 58 is indispensable for proper function of theVirulence · 2022Article
- Bacterial nucleoid-associated protein HU as an extracellular player in host-pathogen interaction.Frontiers in cellular and infection microbiology · 2022Review
- HupB, a nucleoid-associated protein, is critical for survival ofFrontiers in microbiology · 2022Article
- Single-Molecule/Cell Analyses Reveal Principles of Genome-Folding Mechanisms in the Three Domains of Life.International journal of molecular sciences · 2021Review
- A Novel Regulation of K-antigen Capsule Synthesis inFrontiers in oral health · 2021Article
- Structure-based inhibitors targeting the alpha-helical domain of the Spiroplasma melliferum histone-like HU protein.Scientific reports · 2020Article
- Nucleoid-Associated Protein HU: A Lilliputian in Gene Regulation of Bacterial Virulence.Frontiers in cellular and infection microbiology · 2019Review
- HU protein is involved in intracellular growth and full virulence of Francisella tularensis.Virulence · 2018Article
- Natural antigenic differences in the functionally equivalent extracellular DNABII proteins of bacterial biofilms provide a means for targeted biofilm therapeutics.Molecular oral microbiology · 2017Article
- Genes Contributing toFrontiers in cellular and infection microbiology · 2017Article
- Porphyromonas gingivalis: An Overview of Periodontopathic Pathogen below the Gum Line.Frontiers in microbiology · 2016Review
- Synthesis of Sphingolipids Impacts Survival ofFrontiers in microbiology · 2016Article
- Genome-Wide Transcriptional Regulation and Chromosome Structural Arrangement by GalR inFrontiers in molecular biosciences · 2016Article
- Deletion of a 77-base-pair inverted repeat element alters the synthesis of surface polysaccharides in Porphyromonas gingivalis.Journal of bacteriology · 2015Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
HU is a non-sequence-specific DNA-binding protein and one of the most abundant nucleoid-associated proteins in the bacterial cell. Like Escherichia coli, the genome of Porphyromonas gingivalis is predicted to encode both the HUα (PG1258) and the HUβ (PG0121) subunit. We have previously reported that PG0121 encodes a non-specific DNA-binding protein and that PG0121 is co-transcribed with the K-antigen capsule synthesis operon. We also reported that deletion of PG0121 resulted in downregulation of capsule operon expression and produced a P. gingivalis strain that is phenotypically deficient in surface polysaccharide production. Here, we show through complementation experiments in an E. coli MG1655 hupAB double mutant strain that PG0121 encodes a functional HU homologue. Microarray and quantitative RT-PCR analysis were used to further investigate global transcriptional regulation by HUβ using comparative expression profiling of the PG0121 (HUβ) mutant strain to the parent strain, W83. Our analysis determined that expression of genes encoding proteins involved in a variety of biological functions, including iron acquisition, cell division and translation, as well as a number of predicted nucleoid associated proteins were altered in the PG0121 mutant. Phenotypic and quantitative real-time-PCR (qRT-PCR) analyses determined that under iron-limiting growth conditions, cell division and viability were defective in the PG0121 mutant. Collectively, our studies show that PG0121 does indeed encode a functional HU homologue, and HUβ has global regulatory functions in P. gingivalis; it affects not only production of capsular polysaccharides but also expression of genes involved in basic functions, such as cell wall synthesis, cell division and iron uptake.
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