ArticleJournal of clinical microbiology2013
Rapid detection of Panton-Valentine leukocidin in Staphylococcus aureus cultures by use of a lateral flow assay based on monoclonal antibodies.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Molecular typing of PVL-positive Staphylococcus aureus isolates from Lithuania and genome sequencing of a local outbreak strain.Scientific reports · 2026Article
- Rapid Detection of Panton-Valentine Leukocidin Production in Clinical Isolates ofPathogens (Basel, Switzerland) · 2025Article
- Clonal Complexes Distribution ofAntibiotics (Basel, Switzerland) · 2023Article
- Necrotizing Pneumonia in Children: Early Recognition and Management.Journal of clinical medicine · 2023Review
- Characterisation of Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- Exudative Epidermitis in Combination with Staphylococcal Pyoderma in Suckling Piglets.Antibiotics (Basel, Switzerland) · 2021Article
- Characterisation of S. aureus/MRSA CC1153 and review of mobile genetic elements carrying the fusidic acid resistance gene fusC.Scientific reports · 2021Article
- Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin ResistantFrontiers in genetics · 2021Article
- Lateral Flow Immunoassay for the Detection of Panton-Valentine Leukocidin inFrontiers in cellular and infection microbiology · 2021Article
- Clinically Important Toxins in Bacterial Infection: Utility of Laboratory Detection.Clinical microbiology newsletter · 2020Article
- Genotyping of methicillin-resistant Staphylococcus aureus from sepsis patients in Pakistan and detection of antibodies against staphylococcal virulence factors.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Characterisation of a novel SCCmec VI element harbouring fusC in an emerging Staphylococcus aureus strain from the Arabian Gulf region.PloS one · 2019Article
- Molecular Typing of ST239-MRSA-III From Diverse Geographic Locations and the Evolution of the SCCFrontiers in microbiology · 2018Article
- Molecular epidemiology of Staphylococcus aureus from Lambaréné, Gabon.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Increased Community-Associated Infections Caused by Panton-Valentine Leukocidin-Negative MRSA, Shanghai, 2005-2014.Emerging infectious diseases · 2016Article
- Molecular characterization of Staphylococcus aureus isolates causing skin and soft tissue infections in patients from Malakand, Pakistan.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Diversity of SCCmec Elements in Staphylococcus aureus as Observed in South-Eastern Germany.PloS one · 2016Article
- Article
- Direct, Specific and Rapid Detection of Staphylococcal Proteins and Exotoxins Using a Multiplex Antibody Microarray.PloS one · 2015Article
- Molecular typing of MRSA and of clinical Staphylococcus aureus isolates from Iaşi, Romania.PloS one · 2014Article
Corrections and comments
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spotted on protein microarrays and screened using different lukF-PV preparations and detection antibodies. This led to the identification of the optimal antibody combination that was then used to establish a lateral flow assay. This test was used to detect PVL in S. aureus cultures. The detection limit of the assay with purified native and recombinant antigens was determined to be around 1 ng/ml. Overnight cultures from various solid and liquid media proved suitable for PVL detection. Six hundred strains and clinical isolates from patients from America, Europe, Australia, Africa, and the Middle East were tested. Isolates were genotyped in parallel by DNA microarray hybridization for confirmation of PVL status and assignment to clonal complexes. The sensitivity, specificity, and positive and negative predictive values of the assay in this trial were 99.7, 98.3, 98.4, and 99.7%, respectively. A total of 302 clinical isolates and reference strains were PVL positive and were assigned to 21 different clonal complexes. In summary, the lateral flow test allows rapid and economical detection of PVL in a routine bacteriology laboratory. As the test utilizes cultures from standard media and does not require sophisticated equipment, it can be easily integrated into a laboratory's workflow and might contribute to timely therapy of PVL-associated infections.
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