ArticleJournal of clinical microbiology2013
Influence of different media, incubation times, and temperatures for determining the MICs of seven antifungal agents against Paracoccidioides brasiliensis by microdilution.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- Phenotypic characterization of cryptic species in the fungal pathogenbioRxiv : the preprint server for biology · 2024Article
- Fungistatic Effect of Phthalide Lactones onMolecules (Basel, Switzerland) · 2023Article
- An Indian lineage of Histoplasma with strong signatures of differentiation and selection.Fungal genetics and biology : FG & B · 2022Article
- In vitro antifungal susceptibility of Sporothrix globosa isolates from Jilin Province, northeastern China: comparison of yeast and mycelial phases.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Factors influencing susceptibility testing of antifungal drugs: a critical review of document M27-A4 from the Clinical and Laboratory Standards Institute (CLSI).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Review
- Neuroparacoccidioidomycosis: A 13-Year Cohort Study, Rio de Janeiro, Brazil.Journal of fungi (Basel, Switzerland) · 2020Article
- Overview of Antifungal Drugs against Paracoccidioidomycosis: How Do We Start, Where Are We, and Where Are We Going?Journal of fungi (Basel, Switzerland) · 2020Review
- Influence of biofilm growth age, media, antibiotic concentration and exposure time on Staphylococcus aureus and Pseudomonas aeruginosa biofilm removal in vitro.BMC microbiology · 2020Article
- Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment.PLoS neglected tropical diseases · 2019Article
- Exogenous fatty acids alter phospholipid composition, membrane permeability, capacity for biofilm formation, and antimicrobial peptide susceptibility in Klebsiella pneumoniae.MicrobiologyOpen · 2019Article
- Novel Cell Wall Antifungals Reveal a Special Synergistic Activity inFrontiers in microbiology · 2019Article
- Promising New Antifungal Treatment Targeting Chorismate Synthase fromAntimicrobial agents and chemotherapy · 2019Article
- Effects of two 6-quinolinyl chalcones on the integrity of plasma membrane of Paracoccidioides brasiliensis.The Journal of antibiotics · 2017Article
- Antifungal therapeutics for dimorphic fungal pathogens.Virulence · 2017Article
- Paracoccidioides brasiliensis PS2: First Autochthonous Paracoccidioidomycosis Case Report in Rio de Janeiro, Brazil, and Literature Review.Mycopathologia · 2016Article
- Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds.Memorias do Instituto Oswaldo Cruz · 2016Article
- Melanin protects Paracoccidioides brasiliensis from the effects of antimicrobial photodynamic inhibition and antifungal drugs.Antimicrobial agents and chemotherapy · 2015Article
- Identification of New Antifungal Compounds Targeting Thioredoxin Reductase of Paracoccidioides Genus.PloS one · 2015Article
- Fatal fungemia due to Paracoccidioides lutzii.The American journal of tropical medicine and hygiene · 2014Article
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Authors and funding
7 authors.
Funding
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Abstract
MIC assays with Paracoccidioides brasiliensis, the etiological agent of paracoccidioidomycosis, had been conducted with variable protocols, employing both macrodilution and microdilution tests and including differences in inoculum preparation, media used, incubation periods, and temperatures. Twenty-one clinical and environmental isolates of Paracoccidioides were tested using amphotericin B, itraconazole, ketoconazole, fluconazole, sulfamethoxazole, sulfamethoxazole-trimethoprim, and terbinafine, according to the National Committee for Clinical Laboratory Standards (National Committee for Clinical Laboratory Standards, document M27-A2, 2002), with modifications such as three medium formulations (RPMI 1640 medium, McVeigh and Morton [MVM] medium, and modified Mueller-Hinton [MMH] medium), two incubation temperatures (room temperature [25 to 28 °C] and 37 °C), and three incubation periods (7, 10, and 15 days). The antifungal activities were also classified as fungicidal or fungistatic. The best results were obtained after 15 days of incubation, which was chosen as the standard incubation time. The MICs for most individual isolates grown for the same length of time at the same temperature varied with the different media used (P < 0.05). Of the isolates, 81% showed transition from the yeast to the mycelial form in RPMI 1640 medium at 37 °C, independent of the presence of antifungals. MMH medium appears to be a suitable medium for susceptibility testing of antifungal drugs with P. brasiliensis, except for sulfamethoxazole and the combination of sulfamethoxazole-trimethoprim, for which the MVM medium yielded better results. The incubation temperature influenced the MICs, with, in general, higher MICs at 25 °C (mycelial form) than at 37 °C (P < 0.05). Based on our results, we tentatively propose a microdilution assay protocol for susceptibility testing of antifungal drugs against Paracoccidioides.
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