ArticleG3 (Bethesda, Md.)2012
Gal4-based enhancer-trapping in the malaria mosquito Anopheles stephensi.
Article in G3 (Bethesda, Md.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Development of a novel simplified Q-system with integrated temporal expression control.bioRxiv : the preprint server for biology · 2026Article
- Selection, counterselection, and conditional gene expression for genetic biocontrol of insects.Insect science · 2026Review
- History of Mosquito Transgenesis: A Perspective in Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
- An expanded neurogenetic toolkit to decode olfaction in the African malaria mosquito Anopheles gambiae.Cell reports methods · 2024Article
- Mosquito midgut stem cell cellular defense response limits Plasmodium parasite infection.Nature communications · 2024Article
- ImprovedInternational journal of molecular sciences · 2023Article
- Technological advances in mosquito olfaction neurogenetics.Trends in genetics : TIG · 2023Review
- Use of Insect Promoters in Genetic Engineering to Control Mosquito-Borne Diseases.Biomolecules · 2022Review
- Genetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes.Scientific reports · 2022Article
- Identification of new Anopheles gambiae transcriptional enhancers using a cross-species prediction approach.Insect molecular biology · 2021Article
- The regulatory genome of the malaria vectorNAR genomics and bioinformatics · 2021Article
- Review
- Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila.Insect biochemistry and molecular biology · 2020Article
- Leaning Into the Bite: The piRNA Pathway as an Exemplar for the Genetic Engineering Need in Mosquitoes.Frontiers in cellular and infection microbiology · 2020Article
- Chromatin Structure and Function in Mosquitoes.Frontiers in genetics · 2020Review
- Influence of genetic polymorphism on transcriptional enhancer activity in the malaria vector Anopheles coluzzii.Scientific reports · 2019Article
- Molecular tools to create new strains for mosquito sexing and vector control.Parasites & vectors · 2018Review
- Enhancer Trapping and Annotation in Zebrafish Mediated with Sleeping Beauty, piggyBac and Tol2 Transposons.Genes · 2018Article
- Identification of Aedes aegypti cis-regulatory elements that promote gene expression in olfactory receptor neurons of distantly related dipteran insects.Parasites & vectors · 2018Article
- An overview of functional genomic tools in deciphering insecticide resistance.Current opinion in insect science · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Transposon-based forward and reverse genetic technologies will contribute greatly to ongoing efforts to study mosquito functional genomics. A piggyBac transposon-based enhancer-trap system was developed that functions efficiently in the human malaria vector, Anopheles stephensi. The system consists of six transgenic lines of Anopheles stephensi, each with a single piggyBac-Gal4 element in a unique genomic location; six lines with a single piggyBac-UAStdTomato element; and two lines, each with a single Minos element containing the piggyBac-transposase gene under the regulatory control of the hsp70 promoter from Drosophila melanogaster. Enhancer detection depended upon the efficient remobilization of piggyBac-Gal4 transposons, which contain the yeast transcription factor gene Gal4 under the regulatory control of a basal promoter. Gal4 expression was detected through the expression of the fluorescent protein gene tdTomato under the regulatory control of a promoter with Gal4-binding UAS elements. From five genetic screens for larval- and adult-specific enhancers, 314 progeny were recovered from 24,250 total progeny (1.3%) with unique patterns of tdTomato expression arising from the influence of an enhancer. The frequency of piggyBac remobilization and enhancer detection was 2.5- to 3-fold higher in female germ lines compared with male germ lines. A small collection of enhancer-trap lines are described in which Gal4 expression occurred in adult female salivary glands, midgut, and fat body, either singly or in combination. These three tissues play critical roles during the infection of Anopheles stephensi by malaria-causing Plasmodium parasites. This system and the lines generated using it will be valuable resources to ongoing mosquito functional genomics efforts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.