ArticleJournal of lipid research2013
ATPase activity of human ABCG1 is stimulated by cholesterol and sphingomyelin.
Article in Journal of lipid research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- A Structural Bioinformatics-Guided Study of Adenosine Triphosphate-Binding Cassette (ABC) Transporters and Their Substrates.Membranes · 2025Article
- Review
- Exploring bat-inspired cyclic tryptophan diketopiperazines as ABCB1 Inhibitors.Communications chemistry · 2024Article
- Identification of two novel heterodimeric ABC transporters in melanoma: ABCB5β/B6 and ABCB5β/B9.The Journal of biological chemistry · 2024Article
- Is reverse cholesterol transport regulated by active cholesterol?Journal of lipid research · 2023Review
- Snapshots of ABCG1 and ABCG5/G8: A Sterol's Journey to Cross the Cellular Membranes.International journal of molecular sciences · 2022Review
- ATP-Binding Cassette Protein ABCC10 Deficiency Prevents Diet-Induced Obesity but Not Atherosclerosis in Mice.International journal of molecular sciences · 2022Article
- The Reentry Helix Is Potentially Involved in Cholesterol Sensing of the ABCG1 Transporter Protein.International journal of molecular sciences · 2022Article
- Cholesterol efflux mechanism revealed by structural analysis of human ABCA1 conformational states.Nature cardiovascular research · 2022Article
- Transmembrane Polar Relay Drives the Allosteric Regulation for ABCG5/G8 Sterol Transporter.International journal of molecular sciences · 2020Article
- Do ABC transporters regulate plasma membrane organization?Cellular & molecular biology letters · 2020Review
- Article
- Emerging Diversity in Lipid-Protein Interactions.Chemical reviews · 2019Review
- Changes in the asymmetric distribution of cholesterol in the plasma membrane influence streptolysin O pore formation.Scientific reports · 2019Article
- Inward- and outward-facing X-ray crystal structures of homodimeric P-glycoprotein CmABCB1.Nature communications · 2019Article
- Temporary sequestration of cholesterol and phosphatidylcholine within extracellular domains of ABCA1 during nascent HDL generation.Scientific reports · 2018Article
- Sterol Metabolism and Transport in Atherosclerosis and Cancer.Frontiers in endocrinology · 2018Review
- Intracellular and Plasma Membrane Events in Cholesterol Transport and Homeostasis.Journal of lipids · 2018Review
- Critical Role of the Human ATP-Binding Cassette G1 Transporter in Cardiometabolic Diseases.International journal of molecular sciences · 2017Review
- Phosphatidylethanolamine dynamics are required for osteoclast fusion.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ATP-binding cassette protein G1 (ABCG1) is important for the formation of HDL. However, the biochemical properties of ABCG1 have not been reported, and the mechanism of how ABCG1 is involved in HDL formation remains unclear. We established a procedure to express and purify human ABCG1 using the suspension-adapted human cell FreeStyle293-F. ABCG1, fused at the C terminus with green fluorescent protein and Flag-peptide, was solubilized with n-dodecyl-β-D-maltoside and purified via a single round of Flag-M2 antibody affinity chromatography. The purified ABCG1 was reconstituted in liposome of various lipid compositions, and the ATPase activity was analyzed. ABCG1 reconstituted in egg lecithin showed ATPase activity (150 nmol/min/mg), which was inhibited by beryllium fluoride. The ATPase activity of ABCG1, reconstituted in phosphatidylserine liposome, was stimulated by cholesterol and choline phospholipids (especially sphingomyelin), and the affinity for cholesterol was increased by the addition of sphingomyelin. These results suggest that ABCG1 is an active lipid transporter and possesses different binding sites for cholesterol and sphingomyelin, which may be synergistically coupled.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.