ArticleCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology2012
SGK3 regulates Ca(2+) entry and migration of dendritic cells.
Article in Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
- AGC kinase inhibitors regulate STING signaling through SGK-dependent and SGK-independent mechanisms.Cell chemical biology · 2023Article
- Anticancer bioactivity of zerumbone on pediatric rhabdomyosarcoma cells.Journal of cancer research and clinical oncology · 2023Article
- Combination therapy of doxorubicin and Sildenafil inhibits the growth of pediatric rhabdomyosarcoma.Journal of cancer research and clinical oncology · 2023Article
- Ubiquitin D promotes the progression of rheumatoid arthritis via activation of the p38 MAPK pathway.Molecular medicine reports · 2023Article
- Contributions of SGK3 to transporter-related diseases.Frontiers in cell and developmental biology · 2022Review
- Effect of MgClInternational journal of molecular sciences · 2021Article
- Vasopressin-stimulated ORAI1 expression and store-operated CaJournal of molecular medicine (Berlin, Germany) · 2021Article
- Stimulation of ORAI1 expression, store-operated CaPflugers Archiv : European journal of physiology · 2020Article
- Beta-Glycerophosphate-Induced ORAI1 Expression and Store Operated CaScientific reports · 2020Article
- Phosphate-induced ORAI1 expression and store-operated CaJournal of molecular medicine (Berlin, Germany) · 2019Article
- Targeting of SGK1 by miR-576-3p Inhibits Lung Adenocarcinoma Migration and Invasion.Molecular cancer research : MCR · 2019Article
- Lack of serum- and glucocorticoid-inducible kinase 3 leads to podocyte dysfunction.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- SGK3 sustains ERα signaling and drives acquired aromatase inhibitor resistance through maintaining endoplasmic reticulum homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The role of STIM and ORAI proteins in phagocytic immune cells.American journal of physiology. Cell physiology · 2016Review
- Up-regulation of Orai1 expression and store operated Ca(2+) entry following activation of membrane androgen receptors in MCF-7 breast tumor cells.BMC cancer · 2015Article
- Up-regulation of Kv1.3 channels by janus kinase 2.The Journal of membrane biology · 2015Article
- Enhanced Orai1 and STIM1 expression as well as store operated Ca2+ entry in therapy resistant ovary carcinoma cells.Oncotarget · 2014Article
- Down-regulation of the epithelial Na⁺ channel ENaC by Janus kinase 2.The Journal of membrane biology · 2014Article
- Up-regulation of hERG K⁺ channels by B-RAF.PloS one · 2014Article
- Decreased store operated Ca2+ entry in dendritic cells isolated from mice expressing PKB/SGK-resistant GSK3.PloS one · 2014Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
BACKGROUND/
aimsDendritic cells (DCs) are antigen-presenting cells linking innate and adaptive immunity. DC maturation and migration are governed by alterations of cytosolic Ca(2+) concentrations ([Ca(2+)](i)). Ca(2+) entry is in part accomplished by store-operated Ca(2+) (SOC) channels consisting of the membrane pore-forming subunit Orai and the ER Ca(2+) sensing subunit STIM. Moreover, DC functions are under powerful regulation of the phosphatidylinositol-3-kinase (PI3K) pathway, which suppresses proinflammatory cytokine production but supports DC migration. Downstream targets of PI3K include serum- and glucocorticoid-inducible kinase isoform SGK3. The present study explored, whether SGK3 participates in the regulation of [Ca(2+)](i) and Ca(2+)-dependent functions of DCs, such as maturation and migration. METHODS/
resultsExperiments were performed with bone marrow derived DCs from gene targeted mice lacking SGK3 (sgk3(-/-)) and DCs from their wild type littermates (sgk3(+/+)). Maturation, phagocytosis and cytokine production were similar in sgk3(-/-) and sgk3(+/+) DCs. However, SOC entry triggered by intracellular Ca(2+) store depletion with the endosomal Ca(2+) ATPase inhibitor thapsigargin (1 µM) was significantly reduced in sgk3(-/-) compared to sgk3(+/+) DCs. Similarly, bacterial lipopolysaccharide (LPS, 1 µg/ml)- and chemokine CXCL12 (300 ng/ml)- induced increase in [Ca(2+)](i) was impaired in sgk3(-/-) DCs. Moreover, currents through SOC channels were reduced in sgk3(-/-) DCs. STIM2 transcript levels and protein abundance were significantly lower in sgk3(-/-) DCs than in sgk3(+/+) DCs, whereas Orai1, Orai2, STIM1 and TRPC1 transcript levels and/or protein abundance were similar in sgk3-/- and sgk3(+/+) DCs. Migration of both, immature DCs towards CXCL12 and LPS-matured DCs towards CCL21 was reduced in sgk3(-/-) as compared to sgk3(+/+) DCs. Migration of sgk3(+/+) DCs was further sensitive to SOC channel inhibitor 2-APB (50 µM) and to STIM1/STIM2 knock-down.
conclusionSGK3 contributes to the regulation of store-operated Ca(2+) entry into and migration of dendritic cells, effects at least partially mediated through SGK3-dependent upregulation of STIM2 expression.
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Registered trials
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