ArticleBMC plant biology2012
Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species.
Article in BMC plant biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 13 citations in OpenAlex.
- Seed Proteomic Profiles of ThreeBioMed research international · 2020Article
- Allelic variation of low molecular weight glutenin subunits composition and the revealed genetic diversity in durum wheat (Breeding science · 2018Article
- Time-dependent leaf proteome alterations of Brachypodium distachyon in response to drought stress.Plant molecular biology · 2017Article
- Molecular characterization and evolutionary origins of farinin genes in Brachypodium distachyon L.Journal of applied genetics · 2016Article
- Characterisation of low molecular weight glutenin subunit genes from Pseudoroegneria spicata and Pd. strigosa.Journal of applied genetics · 2015Article
- Dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon: comparison with common wheat and Aegilops peregrina.BMC plant biology · 2014Article
- The α-gliadin genes from Brachypodium distachyon L. provide evidence for a significant gap in the current genome assembly.Functional & integrative genomics · 2014Article
- Molecular characterisation and evolution of HMW glutenin subunit genes in Brachypodium distachyon L.Journal of applied genetics · 2014Article
- Cloning and characterization of low-molecular-weight glutenin subunit alleles from Chinese wheat landraces (Triticum aestivum L.).TheScientificWorldJournal · 2014Article
- Molecular characterization and expression profiling of the protein disulfide isomerase gene family in Brachypodium distachyon L.PloS one · 2014Article
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBrachypodium distachyon L. is a newly emerging model plant system for temperate cereal crop species. However, its grain protein compositions are still not clear. In the current study, we carried out a detailed proteomics and molecular genetics study on grain glutenin proteins in B. distachyon.
resultsSDS-PAGE and RP-HPLC analysis of grain proteins showed that Brachypodium has few gliadins and high molecular weight glutenin subunits. In contrast the electrophoretic patterns for the albumin, globulin and low molecular weight glutenin subunit (LMW-GS) fractions of the grain protein were similar to those in wheat. In particular, the LMW-C type subunits in Brachypodium were more abundant than the equivalent proteins in common wheat. Southern blotting analysis confirmed that Brachypodium has 4-5 copies of LMW-GS genes. A total of 18 LMW-GS genes were cloned from Brachypodium by allele specific PCR. LMW-GS and 4 deduced amino acid sequences were further confirmed by using Western-blotting and MALDI-TOF-MS. Phylogenetic analysis indicated that Brachypodium was closer to Ae. markgrafii and Ae. umbellulata than to T. aestivum.
conclusionsBrachypodium possessed a highly conserved Glu-3 locus that is closely related to Triticum and related species. The presence of LMW-GS in B. distachyon grains indicates that B. distachyon may be used as a model system for studying wheat quality attributes.
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